Reference Report

Reference
Citation	Fouquet, W., Owald, D., Wichmann, C., Mertel, S., Depner, H., Dyba, M., Hallermann, S., Kittel, R.J., Eimer, S., Sigrist, S.J. (2009). Maturation of active zone assembly by Drosophila Bruchpilot. J. Cell Biol. 186(1): 129--145. (Export to RIS)
FlyBase ID	FBrf0208461
Publication Type	Research paper
PubMed ID	19596851
PubMed Abstract	Synaptic vesicles fuse at active zone (AZ) membranes where Ca(2+) channels are clustered and that are typically decorated by electron-dense projections. Recently, mutants of the Drosophila melanogaster ERC/CAST family protein Bruchpilot (BRP) were shown to lack dense projections (T-bars) and to suffer from Ca(2+) channel-clustering defects. In this study, we used high resolution light microscopy, electron microscopy, and intravital imaging to analyze the function of BRP in AZ assembly. Consistent with truncated BRP variants forming shortened T-bars, we identify BRP as a direct T-bar component at the AZ center with its N terminus closer to the AZ membrane than its C terminus. In contrast, Drosophila Liprin-alpha, another AZ-organizing protein, precedes BRP during the assembly of newly forming AZs by several hours and surrounds the AZ center in few discrete punctae. BRP seems responsible for effectively clustering Ca(2+) channels beneath the T-bar density late in a protracted AZ formation process, potentially through a direct molecular interaction with intracellular Ca(2+) channel domains.
DOI	10.1083/jcb.200812150
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (2)
	Df(2R)brp^6.1 Df(2R)BSC29
Alleles (19)
	brp^1.3 brp^5.45 brp⁶⁹ brp^c04298 brp^{D1-3.Scer\UAS.T:Avic\GFP-EGFP} brp^{D1-4.Scer\UAS.T:Avic\GFP-EGFP} brp^{D2-4.Scer\UAS.T:Avic\GFP-EGFP} brp^{S.Scer\UAS.T:Avic\GFP-EGFP} brp^{S.Scer\UAS.T:Disc\RFP-mStraw} cac^{Scer\UAS.T:Avic\GFP-EGFP} CG1888^6.1 GluRIIA^{T:Disc\RFP-mRFP} Liprin-α^F3ex15 Liprin-α^R60 Liprin-α^{Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^dpp.blk1 Scer\GAL4^Rapgap1-OK6 T:Avic\GFP^EGFP T:Disc\RFP^mStraw
Constructs (8)
	P{GAL4-dpp.blk1} P{UAS-brp.D1-3-EGFP} P{UAS-brp.D1-4-EGFP} P{UAS-brp.D2-4-EGFP} P{UAS-brp.S-EGFP} P{UAS-brp.S-mStraw} P{UAS-cac1-EGFP} P{UAS-Liprin-α.EGFP}
Genes (8)
	brp cac CG1888 GluRIIA GluRIID Liprin-α Scer\GAL4 Wnt2
Insertions (2)
	P{XP}d03132 PBac{RB}CG1888^e00065
Natural transposons (1)
	P-element