Reference Report

Reference
Citation	Iovino, N., Pane, A., Gaul, U. (2009). miR-184 has multiple roles in Drosophila female germline development. Dev. Cell 17(1): 123--133. (Export to RIS)
FlyBase ID	FBrf0208499
Publication Type	Research paper
PubMed ID	19619497
PubMed Abstract	Posttranscriptional regulation plays a crucial role in germline and early embryonic development, but the underlying mechanisms are only partially understood. Here we report the genetic and molecular analysis of the maternally and zygotically expressed microRNA miR-184 in Drosophila. Loss of miR-184 leads to multiple severe defects during oogenesis and early embryogenesis, culminating in the complete loss of egg production. Using both in vitro and in vivo assays, we characterize the relevant miR-184 targets and target sites for three of the observed phenotypes. miR-184 controls germline stem cell differentiation by tuning the DPP receptor Saxophone, dorsoventral patterning of the egg shell by regulating the gurken transport factor K10, and anteroposterior patterning of the blastoderm by tuning the transcriptional repressor Tramtrack69. Our study highlights the importance of microRNA-mediated regulation in the major developmental transitions of the female germline, and provides insights into several aspects of microRNA function.
DOI	10.1016/j.devcel.2009.06.008
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
ISBN/ISSN
Data from Reference
Alleles (10)
	bam^Δ86 fs(1)K10¹³⁰ mir-184^Scer\UAS.cIa mir-184^Δ sax⁴ sax^{Scer\UAS.T:Ivir\HA1} Scer\GAL4^C587 Scer\GAL4^GR1 Scer\GAL4^{nos.UTR.T:Hsim\VP16} ttk^e11
Constructs (3)
	P{GAL4::VP16-nos.UTR} P{UAS-mir-184.I} P{UAS-sax.HA}
Genes (19)
	bam fs(1)K10 ftz grk gt hb hts kni Kr Mad mir-184 odd run sax Scer\GAL4 tkv tll ttk vas
Insertions (4)
	P{GAL4}C587 P{GawB}GR1 P{XP}d08710 PBac{WH}f05119
Natural transposons (1)
	P-element