Open Close
Reference
Citation
Keleman, K., Micheler, T., VDRC project members, (2009.8.5). RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center 
FlyBase ID
FBrf0208510
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication
The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y+,w3'}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.  Additional information about the vectors used (pKC26 and pKC43) may be found on the VDRC website.
The genomic position of the landing site insertion P{attP,y+,w3'}VIE-260B has been determined to be  2L:22019296 .
Descriptive statements about off-target and multi-target information correspond to the following parameter ranges:
For specificity (off-target effects; s19 = Specificity score)  
   If s19 =< 0.5 
       "Probable off-target effects."
   If 0.5 < s19 =<0.8 
       "Possible off-target effects."
   If 0.8 < s19 < 1  
       "Possible off-target effects (low probability)."
   If s19 = 1, no comment
For multiple on-target genes (K = number of on-target genes)      
   If K > 1 AND s19 > 0.5
       "Targets multiple paralogous genes."
   If K = 1, no comment
DOI
Related Publication(s)
Research paper
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.
Dietzl et al., 2007, Nature 448(7150): 151--156 [FBrf0200691]
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference
    Alleles (9805)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Genes (9514)
    Datasets (1)
    Molecular Constructs (2)
    Sequence Features (10669)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Insertions (9761)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Experimental Tools (1)
    Transgenic Constructs (10670)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.