Reference Report

Reference
Citation	Flaherty, M.S., Zavadil, J., Ekas, L.A., Bach, E.A. (2009). Genome-wide expression profiling in the Drosophila eye reveals unexpected repression of notch signaling by the JAK/STAT pathway. Dev. Dyn. 238(9): 2235--2253. (Export to RIS)
FlyBase ID	FBrf0208590
Publication Type	Research paper
PubMed ID	19504457
PubMed Abstract	Although the JAK/STAT pathway regulates numerous processes in vertebrates and invertebrates through modulating transcription, its functionally relevant transcriptional targets remain largely unknown. With one jak and one stat (stat92E), Drosophila provides a powerful system for finding new JAK/STAT target genes. Genome-wide expression profiling on eye discs in which Stat92E is hyperactivated, revealed 584 differentially regulated genes, including known targets domeless, socs36E, and wingless. Other differentially regulated genes (chinmo, lama, Mo25, Imp-L2, Serrate, Delta) were validated and may represent new Stat92E targets. Genetic experiments revealed that Stat92E cell-autonomously represses Serrate, which encodes a Notch ligand. Loss of Stat92E led to de-repression of Serrate in the dorsal eye, resulting in ectopic Notch signaling and aberrant eye growth there. Thus, our micro-array documents a new Stat92E target gene and a previously unidentified inhibitory action of Stat92E on Notch signaling. These data suggest that this study will be a useful resource for the identification of additional Stat92E targets.
DOI	10.1002/dvdy.21989
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Dyn.
Title	Developmental Dynamics
Publication Year	1992-
ISBN/ISSN	1058-8388
Data from Reference
Alleles (15)
	Avic\GFP^{S65T.Scer\UAS} Avic\GFP^Stat92E.10X Ecol\lacZ^Dl-05151 Ecol\lacZ^eyg-M3-12 Ecol\lacZ^HLHmβ.1.5 Ecol\lacZ^Ser.II-9.5 hop^Scer\UAS.cHa os^GMR.PB os^Scer\UAS.cZa os^unspecified Scer\GAL4^dome-PG14 Scer\GAL4^pnr-MD237 Scer\GAL4^{Scer\FRT.Act5C} Stat92E^85C9 Stat92E³⁹⁷
Constructs (8)
	P{10XStat92E-GFP} P{AyGAL4} P{GMR-upd.B} P{mβ1.5-lacZ} P{Ser-lacZ.II-9.5} P{UAS-GFP.S65T} P{UAS-hop.H} P{UAS-os.Z}
Genes (35)
	Ance aPKC Avic\GFP brat chinmo Dl dlg1 dom dome ds E(spl)mβ-HLH Ecol\lacZ esg fng Fps85D grass GstE13 hop ImpL2 ken lama mirr Mo25 os Pdp1 pnr pnt pyd Scer\GAL4 Ser Socs36E SrpRβ Stat92E trol wg
Insertions (4)
	P{GawB}dome^PG14 P{GawB}pnr^MD237 P{lacW}eyg^M3-12 P{PZ}Dl⁰⁵¹⁵¹