Reference Report

Reference
Citation	Althoff, F., Viktorinová, I., Kastl, J., Lehner, C.F. (2009). Drosophila Cyclin J is a mitotically stable Cdk1 partner without essential functions. Dev. Biol. 333(2): 263--272. (Export to RIS)
FlyBase ID	FBrf0208634
Publication Type	Research paper
PubMed ID	19591820
PubMed Abstract	Cyclin J is a cyclin family member that appears to have evolved before the metazoan radiation. Its evolutionary conservation argues for an important role but functional characterizations of Cyclin J have remained very limited. In Drosophila, Cyclin J is expressed only in females. Using transgenic Drosophila lines expressing Cyclin J versions with N- or C-terminal GFP extensions, we demonstrate that it is expressed exclusively in the germline. After low level expression in all nuclei within the germarium, it gets highly enriched in the germinal vesicle within the oocyte until stage 12 of oogenesis, followed by disappearance after germinal vesicle breakdown before the first meiotic division. Surprisingly, Cyclin J is not required for female fertility. Chromosome segregation during female meiosis, as well as the rapid early embryonic cell cycles after fertilization, occurs normally in the complete absence of Cyclin J. Cyclin J with EGFP fused at either N- or C-terminus binds to Cdk1 and not to Cdk2. However, in contrast to the other known Cdk1 partners, the A- and B-type cyclins, Cyclin J is not degraded during mitosis.
DOI	10.1016/j.ydbio.2009.06.042
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (2)
	Df(3L)AJ14 Df(3L)Exel6095
Alleles (22)
	ald^{T:Avic\GFP-EGFP} armi^+t6 cdc2^{Scer\UAS.T:Hsap\MYC} cdc2c^{Scer\UAS.T:Hsap\MYC} CG14971^+t4.2 CycA⁵ CycA^C8LR1 CycB3³ CycB² CycB³ CycE⁰¹⁶⁷² CycE⁰⁵²⁰⁶ CycE^AR95 CycJ^A138 CycJ^DG29702 CycJ^{g.C-term.T:Avic\GFP-EGFP} CycJ^{g.N-term.T:Avic\GFP-EGFP} CycJ^{Scer\UAS.C.T:Avic\GFP-EGFP} CycJ^{Scer\UAS.N.T:Avic\GFP-EGFP} Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Scer\GAL4^prd.RG1 T:Avic\GFP^EGFP
Constructs (11)
	P{EGFP-Mps1.F} P{GAL4-prd.F} P{gCG14971} P{gCycJ-EGFP} P{gEGFP-CycJ} P{genomic-armi} P{matα4-GAL-VP16} P{UAS-Cdk1-myc} P{UAS-Cdk2-myc} P{UAS-CycJ-EGFP} P{UAS-EGFP-CycJ}
Genes (11)
	ald armi cdc2 cdc2c CG14971 CycA CycB CycB3 CycE CycJ Scer\GAL4
Insertions (2)
	P{wHy}CycJ^DG29702 PBac{5HPw⁺}CycJ^A138
Natural transposons (1)
	P-element