Reference Report

Reference
Citation	Jacobsson, L., Kronhamn, J., Rasmuson-Lestander, A. (2009). The Drosophila Pax6 paralogs have different functions in head development but can partially substitute for each other. Mol. Genet. Genomics 282(3): 217--231. (Export to RIS)
FlyBase ID	FBrf0208653
Publication Type	Research paper
PubMed ID	19484263
PubMed Abstract	There are two Pax6 genes in Drosophila melanogaster; eyeless (ey) and twin-of-eyeless (toy), due to a duplication, which most likely occurred in the insect lineage. They encode transcription factors important for head development. Misexpression of either toy or ey can induce formation of ectopic compound eyes. Toy regulates the ey gene by binding to an eye-specific enhancer in its second intron. However, Toy can induce ectopic eyes also in an ey( - ) background, which indicates a redundancy between the two Pax6 copies in eye formation. To elucidate to what extent these two genes are interchangeable, we first generated toy-Gal4 constructs capable of driving the Pax6 genes in a toy-specific manner. Genetic dissection of the promoter proximal region of toy identified a 1,300-bp region around the canonical transcription start that is sufficient to drive toy expression in embryonic brain and eye primorida and in larval eye-antennal discs. We find that exogenous expression of toy can partially rescue the lethality and eye phenotype caused by lethal mutations in ey and vice versa. We therefore conclude that Toy and Ey, to some extent, can substitute for each other. Nevertheless, the phenotypes of the rescued flies indicate that the two Pax6 genes are specialized to regulate defined structures of the fly head.
DOI	10.1007/s00438-009-0458-2
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Genet. Genomics
Title	Molecular Genetics and Genomics
Publication Year	2001-
ISBN/ISSN	1617-4615
Data from Reference
Alleles (19)
	Avic\GFP^{Scer\UAS.T:SV40\nls2} BacA\p35^Scer\UAS.cHa Ecol\lacZ^Scer\UAS.cBa ey^D ey^J5.71 ey^Scer\UAS.cHa RpL14^{dsRNA.IR.Scer\UAS} Scer\GAL4^ey.PH Scer\GAL4^hs.PB Scer\GAL4^toy1 Scer\GAL4^toy2 Scer\GAL4^toy3 Scer\GAL4^toy4 Scer\GAL4^toy5 Scer\GAL4^toy6 Scer\GAL4^vg.PM toy^G7.39 toy^hdl toy^Scer\UAS.cCa
Constructs (15)
	P{GAL4-ey.H} P{GAL4-Hsp70.PB} P{GAL4-vg.M} P{toy-GAL4.1} P{toy-GAL4.2} P{toy-GAL4.3} P{toy-GAL4.4} P{toy-GAL4.5} P{toy-GAL4.6} P{UAS-ey.H} P{UAS-GFP.nls} P{UAS-lacZ.B} P{UAS-p35.H} P{UAS-RpL14.IR} P{UAS-toy.C}
Genes (8)
	Avic\GFP BacA\p35 Ecol\lacZ elav ey RpL14 Scer\GAL4 toy
Natural transposons (1)
	P-element