Reference Report

Reference
Citation	Agrawal, N., Padmanabhan, N., Hasan, G. (2009). Inositol 1,4,5- trisphosphate receptor function in Drosophila insulin producing cells. PLoS ONE 4(8): e6652. (Export to RIS)
FlyBase ID	FBrf0208703
Publication Type	Research paper
PubMed ID	19680544
PubMed Abstract	The Inositol 1,4,5- trisphosphate receptor (InsP(3)R) is an intracellular ligand gated channel that releases calcium from intracellular stores in response to extracellular signals. To identify and understand physiological processes and behavior that depends on the InsP(3) signaling pathway at a systemic level, we are studying Drosophila mutants for the InsP(3)R (itpr) gene. Here, we show that growth defects precede larval lethality and both are a consequence of the inability to feed normally. Moreover, restoring InsP(3)R function in insulin producing cells (IPCs) in the larval brain rescues the feeding deficit, growth and lethality in the itpr mutants to a significant extent. We have previously demonstrated a critical requirement for InsP(3)R activity in neuronal cells, specifically in aminergic interneurons, for larval viability. Processes from the IPCs and aminergic domain are closely apposed in the third instar larval brain with no visible cellular overlap. Ubiquitous depletion of itpr by dsRNA results in feeding deficits leading to larval lethality similar to the itpr mutant phenotype. However, when itpr is depleted specifically in IPCs or aminergic neurons, the larvae are viable. These data support a model where InsP(3)R activity in non-overlapping neuronal domains independently rescues larval itpr phenotypes by non-cell autonomous mechanisms.
DOI	10.1371/journal.pone.0006652
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Alleles (10)
	Dcr-2^Scer\UAS.cDa Itp-r83A^NIG.1063R Itp-r83A^Scer\UAS.cVa Itp-r83A^sv35 Itp-r83A^ug3 Scer\GAL4^12.1 Scer\GAL4^Act5C.PI Scer\GAL4^Ddc.PL Scer\GAL4^elav-C155 Scer\GAL4^Ilp2.PR
Constructs (6)
	P{Act5C-GAL4} P{Ddc-GAL4.L} P{Ilp2-GAL4.R} P{NIG.1063R} P{UAS-Dcr-2.D} P{UAS-Itp-r83A.V}
Genes (7)
	Dcr-2 Ddc Ilp2 Itp-r83A Lip3 Scer\GAL4 Thor
Insertions (2)
	P{GAL4}12.1 P{GawB}elav^C155