Reference Report

Reference
Citation	Li, C., Vagin, V.V., Lee, S., Xu, J., Ma, S., Xi, H., Seitz, H., Horwich, M.D., Syrzycka, M., Honda, B.M., Kittler, E.L., Zapp, M.L., Klattenhoff, C., Schulz, N., Theurkauf, W.E., Weng, Z., Zamore, P.D. (2009). Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies. Cell 137(3): 509--521. (Export to RIS)
FlyBase ID	FBrf0208736
Publication Type	Research paper
PubMed ID	19395009
PubMed Abstract	Piwi-interacting RNAs (piRNAs) silence transposons in animal germ cells. piRNAs are thought to derive from long transcripts spanning transposon-rich genomic loci and to direct an autoamplification loop in which an antisense piRNA, bound to Aubergine or Piwi protein, triggers production of a sense piRNA bound to the PIWI protein Argonaute3 (Ago3). In turn, the new piRNA is envisioned to produce a second antisense piRNA. Here, we describe strong loss-of-function mutations in ago3, allowing a direct genetic test of this model. We find that Ago3 acts to amplify piRNA pools and to enforce on them an antisense bias, increasing the number of piRNAs that can act to silence transposons. We also detect a second, Ago3-independent piRNA pathway centered on Piwi. Transposons targeted by this second pathway often reside in the flamenco locus, which is expressed in somatic ovarian follicle cells, suggesting a role for piRNAs beyond the germline.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Aberrations (1)
	Df(3L)TTT
Alleles (10)
	AGO3^{Scer\UAS.P\T.T:Zzzz\FLAG,T:Hsap\MYC} AGO3^t1 AGO3^t2 AGO3^t3 aub^HN2 aub^QC42 Scer\GAL4^Act5C.PU Scer\GAL4^{nos.UTR.T:Hsim\VP16} vas^{T:Avic\GFP-EGFP} w^+mC
Constructs (4)
	P{Act5C-GAL4.U} P{GAL4::VP16-nos.UTR} P{UASp-Flag-Myc-AGO3} P{vas-EGFP.N}
Genes (22)
	2SrRNA AGO1 AGO2 AGO3 armi aub CG17454 CG17698 CG40298 CG40470 flam mir-8 Mst40 piRNA piwi Scer\GAL4 Ste Su(Ste) T:Hsap\MYC T:Zzzz\FLAG vas w
Natural transposons (97)
	3S18 17.6 297 412 1360 1731 accord accord2 aurora-element baggins Bari1 blood BS BS3 Burdock Circe copia Cr1a diver diver2 Dm88 Doc Doc2-element Doc3-element F-element flea Fw2 G2 G4 G5 G5A G6 GATE G-element gtwin gypsy gypsy2 gypsy3 gypsy4 gypsy5 gypsy6 gypsy8 gypsy10 gypsy11 gypsy12 Helena HeT-A HMS-Beagle hobo hopper hopper2 Idefix I-element INE-1 invader1 invader2 invader3 invader4 Ivk jockey Juan Max-element McClintock mdg1 mdg3 micropia NOF opus Osvaldo P-element Porto1 Q-element Quasimodo R1-2 R1-element R2-element roo rooA rover Rt1a Rt1b Rt1c S-element springer Stalker Stalker2 Stalker3 Stalker4 Tabor TAHRE TART-A TART-B TART-C transib2 Transpac X-element ZAM