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Gambetta, M.C., Müller, J. (2009.8.18). Complementation behaviour of deficiencies and mutations in the sxc/Ogt region. 
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On 24 Aug 2009, at Mon24 Aug  13:02 , Steven Marygold wrote:
Dear Juerg and Maria Cristina,
Thanks for your mails last week.
I've looked over your points and the new table, and it's clear from your extensive tests that your "Df(2R)NC32" stock is not a deletion: it is a point mutation in a single gene (sxc) and, as such, complements Df(2R)NC219.
But as you allude to, it's difficult to reconcile your data against that of Myster et al: their "Df(2R)NC32" stock reportedly uncovered three loci (l(2)NC130/sxc, l(2)NC12 and l(2)NC47) and overlapped with Df(2R)NC219.
I am left wondering whether the "Df(2R)NC32" you have been using has somehow got mislabelled somewhere along the line from the Peifer lab -> Bloomington -> your lab?  For example, I see from Fig 6 in Myster et al that they recovered 6 alleles of l(2)NC130/sxc, only one of which (l(2)NC130NC95) was deposited at Bloomington. Maybe a second l(2)NC130/sxc allele was mistakingly labelled as "Df(2R)NC32"???
Have you contacted the Peifer lab directly about the discrepancies wrt this stock?
For now, rather than deleting the Df(2R)NC32 record in FlyBase and trying to merge conflicting data into a single new allele record for the sxc7 allele, I plan to retain the Df(2R)NC32 record and its associated data (from the Myster paper) and create a new, separate allele record for the sxc7 allele to accommodate your data.  I will add a note to both records stating that your data supports that sxc7 = Df(2R)NC32, but that discrepancies exist between your data and that of Myster et al which prevent the records from being merged.
Thanks again for your help with this.
--
Steven
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On 17 Aug 2009, at Mon17 Aug  16:56 , Maria Cristina Gambetta wrote:
Dear Steven,
We think that Df(2R)NC32 is not a deletion for the following reasons :
-It complements all of the lethal mutations in the 2R region that I
ordered from Bloomington at the time.
-I saw that it complements l(2)NC47, unlike in Myster et al., 2004 (Genetics).
-The molecular data reveals that it has a point mutation in Ogt.
-Df(2R)NC32 does not have a more severe phenotype than the other sxc alleles (which are also point mutations), in transheterozygous or hemizygous combinations.
I'm sending you in attachment an excel file with the results of
complementation tests that I did (most of this data is not mentioned in our Gambetta, Oktaba and Mueller, 2009 (Science) paper).
Best wishes,
Maria Cristina
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On 11 Aug 2009, at Tue11 Aug  10:33 , Juerg Mueller wrote:
Dear Steven,
Your last point (the Df(2R)NC32 is not a deficiency at all, but a point mutation) was the conclusion that we reached from our analyses.
This conclusion was not only based on the sequencing of the sxc7/Df(2R)NC32 allele but also on the complementation behavior of this allele.  In particular, as described in the supplemental online material, Df(2R)NC32/Df(2R)nap1 transheterozygous animals developed into pharate adults that showed the same phenotype like other sxc/Df(2R)nap1 trans-heterozygotes.
Best wishes,
Juerg
Juerg Mueller PhD
Group leader
Gene Expression Programme
European Molecular Biology Laboratory
Meyerhofstrasse 1
69117 Heidelberg
Germany
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On 11/8/09  10:43 , "Steven Marygold" <sjm41@XXXX> wrote:
Dear Juerg,
I am curating your recent Science paper "Essential Role of the
Glycosyltransferase Sxc/Ogt in Polycomb Repression" for FlyBase.
I have a question about the sxc7 allele.
In the main paper, Figure 1A, you show that sxc7 is a point mutation
(AAA->TAA) in the sxc gene, resulting in K550 changing to a STOP codon.
In the supplementary material, you say that sxc7 equates to Df(2R)N
C32, as described in Myster et al. 2004.  But the Myster paper says that Df(2R)NC32 uncovers three genes: l(2)NC130 (=sxc), l(2)NC12 and l(2)NC47.
Do you know how to resolve these two points?
Is the AAA->TAA point mutation the result of one of the breakpoints of Df(2R)NC32 perhaps?
Or is the Df(2R)NC32 not really a deficiency at all, but a point mutation instead?
Your help would be much appreciated (and get your data into FlyBase
sooner!)
Thanks,
--
Steven Marygold
FlyBase
sjm41@XXXX
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File date: 2009.8.18 ; File size: 28000 ; File format: xls ; File name: Gambetta.2009.8.18.xls
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