Reference Report

Reference
Citation	Pek, J.W., Lim, A.K., Kai, T. (2009). Drosophila maelstrom ensures proper germline stem cell lineage differentiation by repressing microRNA-7. Dev. Cell 17(3): 417--424. (Export to RIS)
FlyBase ID	FBrf0208840
Publication Type	Research paper
PubMed ID	19758565
PubMed Abstract	Nuage is a germline-unique perinuclear structure conserved throughout the animal kingdom. Maelstrom (Mael) is an unusual nuage component, as it is also found in the nucleus. Mael contains a High Mobility Group box, known to mediate DNA binding. We show that Mael nuclear function is required for proper differentiation in the Drosophila germline stem cell (GSC) lineage. In mael mutant testes, transit-amplifying cysts fail to differentiate into primary spermatocytes, instead breaking down into ectopic GSCs and smaller cysts, due to a depletion of Bag-of-marbles (Bam) protein. Mael regulates Bam via repression of miR-7. Mael binds the miR-7 promoter and is required for the local accumulation of HP1 and H3K9me3. miR-7 targets bam directly at its 3'UTR, and a reduction in miR-7 expression can rescue germline differentiation defects found in mael mutants by alleviating Bam repression. We propose that Mael ensures proper differentiation in the GSC lineage by repressing miR-7.
DOI	10.1016/j.devcel.2009.07.017
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (1)
	Df(3L)79E-F
Alleles (17)
	aub^HN2 aub^N11 Avic\GFP^{E.bam.3'UTR.mut.tub} Avic\GFP^{E.bam.3'UTR.tub} Avic\GFP^{EGFP.mir-7.αTub84B} Avic\GFP^{EGFP.αTub84B.B} bam^hs.PO ban^unspecified bl^k08305 Dcr-1^Q1147X loqs^f00791 mael^M391 mael^{Scer\UAS.P\T.T:Zzzz\FLAG} mir-7^EP954 mir-7^Scer\UAS.cLb mir-7^Δ1 Scer\GAL4^nos.PG
Constructs (8)
	P{GAL4-nos.NGT} P{hs-bam.O} P{Tub-EGFP.B} P{tub-EGFP.bam.3'UTR} P{tub-EGFP.bam.mut.3'UTR} P{tub-EGFP.mir-7} P{UAS-mir-7.Lb} P{UASp-FLAG-mael}
Genes (21)
	arm aub Avic\GFP bam ban bl Dcr-1 His3 hts loqs mael mir-7 mir-275 mir-306 mir-317 Scer\GAL4 scra Stat92E Su(var)205 T:Zzzz\FLAG vas
Insertions (1)
	P{EP}bl^EP954
Natural transposons (1)
	P-element