Reference Report

Reference
Citation	Spletter, M.L., Liu, J., Liu, J., Su, H., Giniger, E., Komiyama, T., Quake, S., Luo, L. (2007). Lola regulates Drosophila olfactory projection neuron identity and targeting specificity. Neural Dev. 2(): 14. (Export to RIS)
FlyBase ID	FBrf0208891
Publication Type	Research paper
PubMed ID	17634136
PubMed Abstract	Precise connections of neural circuits can be specified by genetic programming. In the Drosophila olfactory system, projection neurons (PNs) send dendrites to single glomeruli in the antenna lobe (AL) based upon lineage and birth order and send axons with stereotyped terminations to higher olfactory centers. These decisions are likely specified by a PN-intrinsic transcriptional code that regulates the expression of cell-surface molecules to instruct wiring specificity.We find that the loss of longitudinals lacking (lola), which encodes a BTB-Zn-finger transcription factor with 20 predicted splice isoforms, results in wiring defects in both axons and dendrites of all lineages of PNs. RNA in situ hybridization and quantitative RT-PCR suggest that most if not all lola isoforms are expressed in all PNs, but different isoforms are expressed at widely varying levels. Overexpression of individual lola isoforms fails to rescue the lola null phenotypes and causes additional phenotypes. Loss of lola also results in ectopic expression of Gal4 drivers in multiple cell types and in the loss of transcription factor gene lim1 expression in ventral PNs.Our results indicate that lola is required for wiring of axons and dendrites of most PN classes, and suggest a need for its molecular diversity. Expression pattern changes of Gal4 drivers in lola-/- clones imply that lola normally represses the expression of these regulatory elements in a subset of the cells surrounding the AL. We propose that Lola functions as a general transcription factor that regulates the expression of multiple genes ultimately controlling PN identity and wiring specificity.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Neural Dev.
Title	Neural Development
Publication Year	2006-
ISBN/ISSN	1749-8104
Data from Reference
Alleles (12)
	lola^3.8.Scer\UAS lola^4.7.Scer\UAS lola^5D2 lola^e76 lola^L.Scer\UAS lola^ORC4 lola^ORE119 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^GH146 Scer\GAL4^Mz19 Scer\GAL4^NP3529 Scer\GAL4^unspecified
Constructs (5)
	P{GAL4} P{UAS-lola.3.8} P{UAS-lola.4.7} P{UAS-lola.L} P{UAS-mCD8::GFP.L}
Genes (4)
	Lim1 lola Mmus\Cd8a Scer\GAL4
Insertions (9)
	P{GawB}GH146 P{GawB}Mz19 P{GawB}NP3529 P{UAS-lola.3.8}1 P{UAS-lola.4.7}1 P{UAS-lola.4.7}3 P{UAS-lola.L}3a P{UAS-lola.L}3b P{UAS-lola.L}3c
Natural transposons (1)
	P-element