Reference Report

Reference
Citation	Umemori, M., Habara, O., Iwata, T., Maeda, K., Nishinoue, K., Okabe, A., Takemura, M., Takahashi, K., Saigo, K., Ueda, R., Adachi-Yamada, T. (2009). RNAi-Mediated Knockdown Showing Impaired Cell Survival in Drosophila Wing Imaginal Disc. Gene Regul. Syst. Bio. 3(): 11--20. (Export to RIS)
FlyBase ID	FBrf0208974
Publication Type	Research paper
PubMed ID	19838331
PubMed Abstract	The genetically amenable organism Drosophila melanogaster has been estimated to have 14,076 protein coding genes in the genome, according to the flybase release note R5.13 (http://flybase.bio.indiana.edu/static_pages/docs/release_notes.html). Recent application of RNA interference (RNAi) to the study of developmental biology in Drosophila has enabled us to carry out a systematic investigation of genes affecting various specific phenotypes. In order to search for genes supporting cell survival, we conducted an immunohistochemical examination in which the RNAi of 2,497 genes was independently induced within the dorsal compartment of the wing imaginal disc. Under these conditions, the activities of a stress-activated protein kinase JNK (c-Jun N-terminal kinase) and apoptosis-executing factor Caspase-3 were monitored. Approximately half of the genes displayed a strong JNK or Caspase-3 activation when their RNAi was induced. Most of the JNK activation accompanied Caspase-3 activation, while the opposite did not hold true. Interestingly, the area activating Caspase-3 was more broadly seen than that activating JNK, suggesting that JNK is crucial for induction of non-autonomous apoptosis in many cases. Furthermore, the RNAi of essential factors commonly regulating transcription and translation showed a severe and cell-autonomous apoptosis but also elicited another apoptosis at an adjacent area in a non-autonomous way. We also found that the frequency of apoptosis varies depending on the tissues.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Gene Regul. Syst. Bio.
Title	Gene regulation and systems biology
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ISBN/ISSN	1177-6250
Data from Reference
Alleles (17)
	aop^NIG.3166R Ark^{dsRNA.Scer\UAS.cLa} Avic\GFP^Scer\UAS.cUa CG14072^NIG.14072R Dad^Scer\UAS.cTa E(spl)m5-HLH^NIG.6096R Ecol\lacZ^puc-E69 Mad^NIG.12399R RpL17^NIG.3203R rpr^Scer\UAS.C RpS14a^NIG.1524R Scer\GAL4^ap-md544 Scer\GAL80^ts.αTub84B shg^{dsRNA.Scer\UAS} Smyd4^NIG.14122R Taf6^NIG.9348R th^{dsRNA.Scer\UAS.cLb}
Constructs (15)
	P{NIG.1524R} P{NIG.3166R} P{NIG.3203R} P{NIG.6096R} P{NIG.9348R} P{NIG.12399R} P{NIG.14072R} P{NIG.14122R} P{tubP-GAL80^ts} P{UAS-Ark.IR.L} P{UAS-Dad.T} P{UAS-GFP.U} P{UAS-rpr.C} P{UAS-shg.IR} P{UAS-th.IR.L}
Genes (21)
	aop ap Ark Avic\GFP bsk CG14072 Dad E(spl)m5-HLH Ecol\lacZ Ice Mad puc RpL17 rpr RpS14a Scer\GAL4 Scer\GAL80 shg Smyd4 Taf6 th
Insertions (2)
	P{A92}puc^E69 P{GawB}ap^md544
Natural transposons (1)
	P-element