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Margueron, R., Justin, N., Ohno, K., Sharpe, M.L., Son, J., Drury, W.J., Voigt, P., Martin, S.R., Taylor, W.R., De Marco, V., Pirrotta, V., Reinberg, D., Gamblin, S.J. (2009). Role of the polycomb protein EED in the propagation of repressive histone marks.  Nature 461(7265): 762--767.
FlyBase ID
FBrf0208990
Publication Type
Research paper
Abstract

Polycomb group proteins have an essential role in the epigenetic maintenance of repressive chromatin states. The gene-silencing activity of the Polycomb repressive complex 2 (PRC2) depends on its ability to trimethylate lysine 27 of histone H3 (H3K27) by the catalytic SET domain of the EZH2 subunit, and at least two other subunits of the complex: SUZ12 and EED. Here we show that the carboxy-terminal domain of EED specifically binds to histone tails carrying trimethyl-lysine residues associated with repressive chromatin marks, and that this leads to the allosteric activation of the methyltransferase activity of PRC2. Mutations in EED that prevent it from recognizing repressive trimethyl-lysine marks abolish the activation of PRC2 in vitro and, in Drosophila, reduce global methylation and disrupt development. These findings suggest a model for the propagation of the H3K27me3 mark that accounts for the maintenance of repressive chromatin domains and for the transmission of a histone modification from mother to daughter cells.

PubMed ID
PubMed Central ID
PMC3772642 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nature
    Title
    Nature
    Publication Year
    1869-
    ISBN/ISSN
    0028-0836
    Data From Reference
    Alleles (6)
    Genes (8)
    Physical Interactions (4)
    Natural transposons (1)
    Experimental Tools (1)
    Transgenic Constructs (4)