Reference Report

Reference
Citation	Barber, C.F., Jorquera, R.A., Melom, J.E., Littleton, J.T. (2009). Postsynaptic regulation of synaptic plasticity by synaptotagmin 4 requires both C2 domains. J. Cell Biol. 187(2): 295--310. (Export to RIS)
FlyBase ID	FBrf0208997
Publication Type	Research paper
PubMed ID	19822673
PubMed Abstract	Ca(2+) influx into synaptic compartments during activity is a key mediator of neuronal plasticity. Although the role of presynaptic Ca(2+) in triggering vesicle fusion though the Ca(2+) sensor synaptotagmin 1 (Syt 1) is established, molecular mechanisms that underlie responses to postsynaptic Ca(2+) influx remain unclear. In this study, we demonstrate that fusion-competent Syt 4 vesicles localize postsynaptically at both neuromuscular junctions (NMJs) and central nervous system synapses in Drosophila melanogaster. Syt 4 messenger RNA and protein expression are strongly regulated by neuronal activity, whereas altered levels of postsynaptic Syt 4 modify synaptic growth and presynaptic release properties. Syt 4 is required for known forms of activity-dependent structural plasticity at NMJs. Synaptic proliferation and retrograde signaling mediated by Syt 4 requires functional C2A and C2B Ca(2+)-binding sites, as well as serine 284, an evolutionarily conserved substitution for a key Ca(2+)-binding aspartic acid found in other synaptotagmins. These data suggest that Syt 4 regulates activity-dependent release of postsynaptic retrograde signals that promote synaptic plasticity, similar to the role of Syt 1 as a Ca(2+) sensor for presynaptic vesicle fusion.
DOI	10.1083/jcb.200903098
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (1)
	Df(3R)rn¹⁶
Alleles (20)
	para^ts1 Scer\GAL4^D42 Scer\GAL4^Mhc.PW sei¹ Syt1^AD4 Syt1^N13 Syt1^{Scer\UAS.T:Avic\GFP-EGFP} Syt4^{3.Scer\UAS.T:Disc\RFP-mRFP} Syt4^{5.Scer\UAS.T:Avic\GFP-YFP} Syt4^BA1 Syt4^{D292N.D427N.D429N.Scer\UAS} Syt4^{D292N.Scer\UAS} Syt4^{D427N.D429N.Scer\UAS} Syt4^{S284A.Scer\UAS} Syt4^{S284D.Scer\UAS} Syt4^{Scer\UAS.T:Avic\GFP-pHluorinE} Syt4^wt.Scer\UAS Syx1A^3-69 T:Avic\GFP^YFP T:Disc\RFP^mRFP
Constructs (11)
	P{GAL4-Mhc.W} P{UAS-Syt4.D292N.D427N.D429N} P{UAS-Syt4.D292N} P{UAS-Syt4.D427N.D429N} P{UAS-Syt4.S284A} P{UAS-Syt4.S284D} P{UAS-Syt4.wt} P{UAS-Syt4-mRFP} P{UAS-syt.eGFP} P{UAS-SytIV-pHluorin} P{UAS-YFP-Syt4}
Genes (9)
	brp cpx dlg1 para Scer\GAL4 sei Syt1 Syt4 Syx1A
Insertions (2)
	P{EPgy2}EY09259 P{GawB}D42
Natural transposons (1)
	P-element