Reference Report

Reference
Citation	Kurshan, P.T., Oztan, A., Schwarz, T.L. (2009). Presynaptic alpha(2)delta-3 is required for synaptic morphogenesis independent of its Ca(2+)-channel functions. Nat. Neurosci. 12(11): 1415--1423. (Export to RIS)
FlyBase ID	FBrf0209005
Publication Type	Research paper
PubMed ID	19820706
PubMed Abstract	Synaptogenesis involves the transformation of a growth cone into synaptic boutons specialized for transmitter release. In Drosophila embryos lacking the alpha(2)delta-3 subunit of presynaptic, voltage-dependent Ca(2+) channels, we found that motor neuron terminals failed to develop synaptic boutons and cytoskeletal abnormalities arose, including the loss of ankyrin2. Nevertheless, functional presynaptic specializations were present and apposed to clusters of postsynaptic glutamate receptors. The alpha(2)delta-3 protein has been thought to function strictly as an auxiliary subunit of the Ca(2+) channel, but the phenotype of alpha(2)delta-3 (also known as stj) mutations cannot be explained by a channel defect; embryos lacking the pore-forming alpha(1) subunit cacophony formed boutons. The synaptogenic function of alpha(2)delta-3 required only the alpha(2) peptide, whose expression sufficed to rescue bouton formation. Our results indicate that alpha(2)delta proteins have functions that are independent of their roles in the biophysics and localization of Ca(2+) channels and that synaptic architecture depends on these functions.
DOI	10.1038/nn.2417
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Neurosci.
Title	Nature Neuroscience
Publication Year	1998-
ISBN/ISSN	1097-6256
Data from Reference
Aberrations (2)
	Df(2L)BSC9 Df(2R)Exel7128
Alleles (13)
	cac^20-3 cac^HC129 cac^{Scer\UAS.T:Avic\GFP-EGFP} Ca-α1D^X10 Scer\GAL4^da.G32 Scer\GAL4^elav-C155 stj^DD106 stj^DD131 stj^DD173 stj^DD174 stj^DD196 stj^{Scer\UAS.Int.T:Ivir\HA1} stj^{Δδ-3.Scer\UAS.T:Ivir\HA1}
Constructs (4)
	P{GAL4-da.G32} P{UAS-cac1-EGFP} PBac{UAS-HA-stj.Δδ-3} PBac{UAS-HA-stj}
Genes (17)
	Ank2 brp cac CadN Ca-α1D Csp dlg1 Fas2 futsch GluRIIA GluRIIC Nrg Scer\GAL4 stj Syt1 T:Ivir\HA1 unc-104
Insertions (1)
	P{GawB}elav^C155
Natural transposons (1)
	Tni\piggyBac