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Citation
Lee, J.Y., Bhatt, D., Bhatt, D., Chung, W.Y., Cooper, R.L. (2009). Furthering pharmacological and physiological assessment of the glutamatergic receptors at the Drosophila neuromuscular junction.  Comp. Biochem. Physiol. C. Toxicol. Pharmacol. 150(4): 546--557.
FlyBase ID
FBrf0209026
Publication Type
Research paper
Abstract

Drosophila melanogaster larval neuromuscular junctions (NMJs) serve as a model for synaptic physiology. The molecular sequences of the postsynaptic glutamate receptors have been described; however, the pharmacological profile has not been fully elucidated. The postsynaptic molecular sequence suggests a novel glutamate receptor subtype. Kainate does not depolarize the muscle, but dampens evoked EPSP amplitudes. Quantal responses show a decreased amplitude and area under the voltage curve indicative of reduced postsynaptic receptor sensitivity to glutamate transmission. ATPA, a kainate receptor agonist, did not mimic kainate's action. The metabotropic glutamate receptor agonist t-ACPD had no effect. Domoic acid, a kainate/AMPA receptor agonist, blocks the postsynaptic receptors without depolarizing the muscle. However, SYM 2081, a kainate receptor agonist, did depolarize the muscle and reduce the EPSP amplitude at 1 mM but not at 0.1 mM. This supports the notion that these are generally a quisqualate subtype receptors with some oddities in the pharmacological profile. The results suggest a direct postsynaptic action of kainate due to partial antagonist action on the quisqualate receptors. There does not appear to be presynaptic auto-regulation via a kainate receptor subtype or a metabotropic auto-receptor. This study aids in furthering the pharmokinetic profiling and specificity of the receptor subtypes.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Comp. Biochem. Physiol. C. Toxicol. Pharmacol.
    Title
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
    Publication Year
    2000--
    ISBN/ISSN
    1532-0456
    Data From Reference