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Citation
Kemppainen, E., Fern├índez-Ayala, D.J., Galbraith, L.C., O'Dell, K.M., Jacobs, H.T. (2009). Phenotypic suppression of the Drosophila mitochondrial disease-like mutant tko(25t) by duplication of the mutant gene in its natural chromosomal context.  Mitochondrion 9(5): 353--363.
FlyBase ID
FBrf0209036
Publication Type
Research paper
Abstract

A mutation in the Drosophila gene technical knockout (tko(25t)), encoding mitoribosomal protein S12, phenocopies human mitochondrial disease. We isolated three spontaneous X-dominant suppressors of tko(25t) (designated Weeble), exhibiting almost wild-type phenotype and containing overlapping segmental duplications including the mutant allele, plus a second mitoribosomal protein gene, mRpL14. Ectopic, expressed copies of tko(25t) and mRpL14 conferred no phenotypic suppression. When placed over a null allele of tko, Weeble retained the mutant phenotype, even in the presence of additional transgenic copies of tko(25t). Increased mutant gene dosage can thus compensate the mutant phenotype, but only when located in its normal chromosomal context.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mitochondrion
    Title
    Mitochondrion
    Publication Year
    2001-
    ISBN/ISSN
    1567-7249
    Data From Reference
    Aberrations (6)
    Alleles (4)
    Genes (13)
    Human Disease Models (1)
    Natural transposons (1)
    Transgenic Constructs (2)