Reference Report

Reference
Citation	Yan, D., Wu, Y., Feng, Y., Lin, S.C., Lin, X. (2009). The core protein of glypican Dally-like determines its biphasic activity in wingless morphogen signaling. Dev. Cell 17(4): 470--481. (Export to RIS)
FlyBase ID	FBrf0209080
Publication Type	Research paper
PubMed ID	19853561
PubMed Abstract	Dally-like (Dlp) is a glypican-type heparan sulfate proteoglycan (HSPG), containing a protein core and attached glycosaminoglycan (GAG) chains. In Drosophila wing discs, Dlp represses short-range Wingless (Wg) signaling, but activates long-range Wg signaling. Here, we show that Dlp core protein has similar biphasic activity as wild-type Dlp. Dlp core protein can interact with Wg; the GAG chains enhance this interaction. Importantly, we find that Dlp exhibits a biphasic response, regardless of whether its glycosylphosphatidylinositol linkage to the membrane can be cleaved. Rather, the transition from signaling activator to repressor is determined by the relative expression levels of Dlp and the Wg receptor, Frizzled (Fz) 2. Based on these data, we propose that the principal function of Dlp is to retain Wg on the cell surface. As such, it can either compete with the receptor or provide ligands to the receptor, depending on the ratios of Wg, Fz2, and Dlp.
DOI	10.1016/j.devcel.2009.09.001
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Supplementary material	Supplemental Data. [FBrf0211306]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Alleles (23)
	dlp::fz2^{Scer\UAS.dlpN.fz2C} dlp^A187 dlp^{GPI.Scer\UAS.T:Avic\GFP-EGFP} dlp^{-HS.Scer\UAS.T:Avic\GFP-EGFP,T:Rnor\CD2} dlp^{-HS.Scer\UAS.T:Avic\GFP-EGFP} dlp^{-HS.Scer\UAS.T:Rnor\CD2} dlp^-HS.Scer\UAS dlp^{N.Scer\UAS.T:Rnor\CD2} dlp^Scer\UAS.cBa dlp^{Scer\UAS.T:Avic\GFP,T:Rnor\CD2} dlp^{Scer\UAS.T:Avic\GFP} dlp^{Scer\UAS.T:SS-wg,T:Avic\GFP} dlp^{ΔGAG.Scer\UAS} dlp^{ΔGPI.Scer\UAS} dlp^ΔN.Scer\UAS fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} fz2^Scer\UAS.cZa fz2^{ΔCRD.Scer\UAS} Scer\GAL4^dpp.blk1 Scer\GAL4^en-e16E Scer\GAL4^hh-Gal4 Scer\GAL80^ts.αTub84B Sdc^Scer\UAS.cJa
Constructs (19)
	P{GAL4-dpp.blk1} P{tubP-GAL80^ts} P{UAS-dlp.GFP} P{UAS-dlp.-HS.CD2} P{UAS-dlp.-HS.GFP.CD2} P{UAS-dlp.-HS.GFP} P{UAS-dlp.-HS} P{UAS-dlp.N-CD2} P{UAS-dlp.WT} P{UAS-dlp.ΔGAG} P{UAS-dlp.ΔGPI} P{UAS-dlp.ΔN} P{UAS-dlpN-fz2C} P{UAS-fz2.ΔCRD} P{UAS-fz2(ECD)-GPI} P{UAS-GFP::dlp} P{UAS-GFP-dlp-CD2} P{UAS-GFP-GPI} P{UAS-Sdc.J}
Genes (11)
	ci Con Dll dlp dlp::fz2 fz2 Scer\GAL4 Scer\GAL80 Sdc sens wg
Insertions (2)
	P{en2.4-GAL4}e16E P{GAL4}hh^Gal4