Reference Report

Reference
Citation	Mao, Y., Kucuk, B., Irvine, K.D. (2009). Drosophila lowfat, a novel modulator of Fat signaling. Development 136(19): 3223--3233. (Export to RIS)
FlyBase ID	FBrf0209094
Publication Type	Research paper
PubMed ID	19710173
PubMed Abstract	The Fat-Hippo-Warts signaling network regulates both transcription and planar cell polarity. Despite its crucial importance to the normal control of growth and planar polarity, we have only a limited understanding of the mechanisms that regulate Fat. We report here the identification of a conserved cytoplasmic protein, Lowfat (Lft), as a modulator of Fat signaling. Drosophila Lft, and its human homologs LIX1 and LIX1-like, bind to the cytoplasmic domains of the Fat ligand Dachsous, the receptor protein Fat, and its human homolog FAT4. Lft protein can localize to the sub-apical membrane in disc cells, and this membrane localization is influenced by Fat and Dachsous. Lft expression is normally upregulated along the dorsoventral boundary of the developing wing, and is responsible for elevated levels of Fat protein there. Levels of Fat and Dachsous protein are reduced in lft mutant cells, and can be increased by overexpression of Lft. lft mutant animals exhibit a wing phenotype similar to that of animals with weak alleles of fat, and lft interacts genetically with both fat and dachsous. These studies identify Lft as a novel component of the Fat signaling pathway, and the Lft-mediated elevation of Fat levels as a mechanism for modulating Fat signaling.
DOI	10.1242/dev.036152
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(2L)ED94
Alleles (21)
	ds^GD14350 ds^UAO71 ft¹ Hsap\LIX1^{Scer\UAS.T:Zzzz\FLAG} Hsap\LIX1L^{Scer\UAS.T:Zzzz\FLAG} lft⁰⁴⁵¹ lft¹⁹²⁵ lft²¹⁰¹ lft³⁷⁰⁹ lft³⁷⁶² lft⁴¹⁶⁸ lft⁴⁹⁰⁷ lft^NIG.13139R lft^{Scer\SceI.RS.cMa} lft^{Scer\UAS.T:Zzzz\FLAG} lft^TG2 Scer\GAL4^Act.PU Scer\GAL4^en-e16E Scer\GAL4^ptc-559.1 Scer\GAL4^tub.PU w^+mW.hs
Constructs (9)
	P{Act-GAL4.U} P{FRT(lft^{Scer\SceI.RS.cMa})} P{GD14350} P{NIG.13139R} P{tub-GAL4.U} P{UAS-lft.FLAG.attB} P{UAS-lft.FLAG} P{UAS-LIX1.FLAG.attB} P{UAS-LIX1L.FLAG.attB}
Genes (14)
	ds ex ft Hsap\LIX1 Hsap\LIX1L lft N Scer\GAL4 shg T:Zzzz\FLAG th w wg wts
Insertions (9)
	P{en2.4-GAL4}e16E P{GawB}ptc^559.1 P{UAS-lft.FLAG.attB}attP2 P{UAS-lft.FLAG}6 P{UAS-lft.FLAG}F P{UAS-lft.FLAG}G P{UAS-lft.FLAG}H P{UAS-LIX1.FLAG.attB}attP2 P{UAS-LIX1L.FLAG.attB}attP2
Natural transposons (1)
	P-element