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Citation
Meyer, H., Panz, M., Zmojdzian, M., Jagla, K., Paululat, A. (2009). Neprilysin 4, a novel endopeptidase from Drosophila melanogaster, displays distinct substrate specificities and exceptional solubility states.  J. Exp. Biol. 212(22): 3673--3683.
FlyBase ID
FBrf0209227
Publication Type
Research paper
Abstract

Proteins belonging to the family of neprilysins are typically membrane bound M13 endopeptidases responsible for the inactivation and/or activation of peptide signaling events on cell surfaces. Mammalian neprilysins are known to be involved in the metabolism of various regulatory peptides especially in the nervous, immune, cardiovascular and inflammatory systems. Although there is still much to learn about their participation in various diseases, they are potential therapeutic targets. Here we report on the identification and first characterization of neprilysin 4 (NEP4) from Drosophila melanogaster. Reporter lines as well as in situ hybridization combined with immunolocalization demonstrated NEP4 expression during embryogenesis in pericardial cells, muscle founder cells, glia cells and male gonads. Western blot analysis confirmed the prediction of one membrane bound and one soluble isoform, a finding quite unusual among neprilysins with presumably strong physiological relevance. At least one NEP4 isoform was found in every developmental stage indicating protein activities required throughout the whole life cycle of Drosophila. Heterologously expressed NEP4 exhibited substrate preferences comparable to human neprilysin 2 with distinct cleavage of substance P and angiotensin I.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Exp. Biol.
    Title
    Journal of Experimental Biology
    Publication Year
    1930-
    ISBN/ISSN
    0022-0949
    Data From Reference
    Alleles (2)
    Genes (8)
    Polypeptides (1)
    Natural transposons (1)
    Experimental Tools (2)
    Transgenic Constructs (2)