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Reference
Citation
Venken, K.J.T., Carlson, J.W., Schulze, K.L., Pan, H., He, Y., Spokony, R., Wan, K.H., Koriabine, M., de Jong, P.J., White, K.P., Bellen, H.J., Hoskins, R.A. (2009). Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster.  Nat. Methods 6(6): 431--434.
FlyBase ID
FBrf0209231
Publication Type
Research paper
Abstract
We constructed Drosophila melanogaster bacterial artificial chromosome libraries with 21-kilobase and 83-kilobase inserts in the P[acman] system. We mapped clones representing 12-fold coverage and encompassing more than 95% of annotated genes onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using UC31 integrase to rescue mutations. They can be modified through recombineering, for example, to incorporate protein tags and assess expression patterns.
PubMed ID
PubMed Central ID
PMC2784134 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Methods
    Title
    Nature Methods
    Publication Year
    2004-
    ISBN/ISSN
    1548-7091 1548-7105
    Data From Reference
    Aberrations (3)
    Alleles (58)
    Clones (49422)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Genes (76)
    Datasets (5)
    Molecular Constructs (1)
    Natural transposons (2)
    Insertions (59)
    Experimental Tools (1)
    Transgenic Constructs (59)