Reference Report

Reference
Citation	Guha, A., Lin, L., Kornberg, T.B. (2009). Regulation of Drosophila matrix metalloprotease Mmp2 is essential for wing imaginal disc:trachea association and air sac tubulogenesis. Dev. Biol. 335(2): 317--326. (Export to RIS)
FlyBase ID	FBrf0209232
Publication Type	Research paper
PubMed ID	19751719
PubMed Abstract	The Drosophila Dorsal Air Sac Primordium (ASP) is a tracheal tube that grows toward Branchless FGF-expressing cells in the wing imaginal disc. We show that the ASP arises from a tracheal branch that invades the basal lamina of the disc to juxtapose directly with disc cells. We examined the role of matrix metalloproteases (Mmps), and found that reducing Mmp2 activity perturbed disc-trachea association, altered peritracheal distributions of collagen IV and Perlecan, misregulated ASP growth, and abrogated development of the dorsal air sacs. Whereas the function of the membrane-tethered Mmp2 in the ASP is non-cell autonomous we find that it may have distinct tissue-specific roles in the ASP and disc. These findings demonstrate a critical role for Mmp2 in tubulogenesis post-induction, and implicate Mmp2 in regulating dynamic and essential changes to the extracellular matrix.
DOI	10.1016/j.ydbio.2009.09.005
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (22)
	Avic\GFP^{Scer\UAS.T:Hsap\Myr2} Avic\GFP^{Scer\UAS.T:SV40\nls2} Avic\GFP^trol-G00022 Avic\GFP^trol-ZCL1700 Avic\GFP^trol-ZCL1973 Avic\GFP^vkg-G00454 bnl^hs.PS Disc\RFP^{DsRed2.Scer\UAS} Mmp1^{dsRNA.Scer\UAS} Mmp1^Q112stop Mmp2^{dsRNA.Scer\UAS} Mmp2^k07511 Mmp2^Scer\UAS.cPa Mmp2^W307stop Scer\GAL4^ap-md544 Scer\GAL4^btl.PS Scer\GAL4^Mmp2-NP0509 Timp^Scer\UAS.cPa trol^G00022 trol^ZCL1700 trol^ZCL1973 vkg^G00454
Constructs (10)
	P{GAL4-btl.S} P{hs-bnl.S} P{PTT-un1} P{UAS-Disc\RFP.DsRed2} P{UAS-gapGFP} P{UAS-GFP.nls} P{UAS-Mmp1.RNAi} P{UAS-Mmp2.P} P{UAS-Mmp2.RNAi} P{UAS-Timp.P}
Genes (13)
	ap Avic\GFP bnl btl Disc\RFP dlg1 Mmp1 Mmp2 rl Scer\GAL4 Timp trol vkg
Insertions (6)
	P{GawB}ap^md544 P{GawB}Mmp2^NP0509 P{PTT-un1}trol^G00022 P{PTT-un1}trol^ZCL1700 P{PTT-un1}trol^ZCL1973 P{PTT-un1}vkg^G00454