Reference Report

Reference
Citation	Prakash, S., McLendon, H.M., Dubreuil, C.I., Ghose, A., Hwa, J., Dennehy, K.A., Tomalty, K.M., Clark, K.L., Van Vactor, D., Clandinin, T.R. (2009). Complex interactions amongst N-cadherin, DLAR, and Liprin-alpha regulate Drosophila photoreceptor axon targeting. Dev. Biol. 336(1): 10--19. (Export to RIS)
FlyBase ID	FBrf0209258
Publication Type	Research paper
PubMed ID	19766621
PubMed Abstract	The formation of stable adhesive contacts between pre- and post-synaptic neurons represents the initial step in synapse assembly. The cell adhesion molecule N-cadherin, the receptor tyrosine phosphatase DLAR, and the scaffolding molecule Liprin-alpha play critical, evolutionarily conserved roles in this process. However, how these proteins signal to the growth cone and are themselves regulated remains poorly understood. Using Drosophila photoreceptors (R cells) as a model, we evaluate genetic and physical interactions among these three proteins. We demonstrate that DLAR function in this context is independent of phosphatase activity but requires interactions mediated by its intracellular domain. Genetic studies reveal both positive and, surprisingly, inhibitory interactions amongst all three genes. These observations are corroborated by biochemical studies demonstrating that DLAR physically associates via its phosphatase domain with N-cadherin in Drosophila embryos. Together, these data demonstrate that N-cadherin, DLAR, and Liprin-alpha function in a complex to regulate adhesive interactions between pre- and post-synaptic cells and provide a novel mechanism for controlling the activity of Liprin-alpha in the developing growth cone.
DOI	10.1016/j.ydbio.2009.09.016
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (17)
	CadN^Scer\UAS.cIa CadN^Δ14 Ecol\lacZ^HLHmδ.0.5 Lar⁴⁵¹ Lar²¹²⁷ Lar^bypass Lar^{C1929S.Scer\UAS} Lar^{CSX2.Scer\UAS} Lar^Scer\UAS.cKa Lar^{ΔFn2-9.Scer\UAS} Lar^{ΔIg123.Scer\UAS} Liprin-α¹ Liprin-α^E Liprin-α^F Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^elav-C155 Scer\GAL4^HLHmδ.0.5
Constructs (9)
	P{HLHmδ-lacZ.0.5} P{mδ0.5-GAL4} P{UAS-CadN.I} P{UAS-Lar.C1929S} P{UAS-Lar.CSX2} P{UAS-Lar.K} P{UAS-Lar.ΔFn2-9} P{UAS-Lar.ΔIg123} P{UAS-mCD8::GFP.L}
Genes (8)
	CadN E(spl)mδ-HLH Ecol\lacZ elav Lar Liprin-α Mmus\Cd8a Scer\GAL4
Insertions (1)
	P{GawB}elav^C155