Reference Report

Reference
Citation	Loya, C.M., Lu, C.S., Van Vactor, D., Fulga, T.A. (2009). Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms. Nat. Methods 6(12): 897--903. (Export to RIS)
FlyBase ID	FBrf0209364
Publication Type	Research paper
PubMed ID	19915559
PubMed Abstract	MicroRNAs are important regulators of gene expression, yet the functional outputs of most microRNA-target interactions remain elusive. Here we introduce the Drosophila melanogaster microRNA sponge (miR-SP) as a powerful transgenic technology to dissect the function of every microRNA with precise spatiotemporal resolution. miR-SPs can be used to characterize tissue-specific microRNA loss-of-function phenotypes, define the spatial regulation of their effectors and uncover interactions between microRNAs and other genes. Using themiR-SP system, we identified an essential role of the conserved microRNA miR-8, in neuromuscular junction formation. Tissue-specific silencing revealed that postsynaptic activity of miR-8 is important for normal neuromuscular junction morphogenesis. Given that miR-SPs rely on a bipartite modular expression system, they could be used to elucidate the endogenous function of microRNAs in any species in which conditional expression can be achieved.
DOI	10.1038/nmeth.1402
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Methods
Title	Nature Methods
Publication Year	2004-
ISBN/ISSN	1548-7091 1548-7105
Data from Reference
Alleles (26)
	aop^ACT.GMR Avic\GFP^{EGFP.mir-8.αTub84B} Avic\GFP^{EGFP.nerfin-1.3'UTR.αTub84B} Avic\GFP^{EGFP.Scer\UAS.mir-8SP} Avic\GFP^{EGFP.Scer\UAS-mir-7SP} Avic\GFP^{EGFP.Scer\UAS-mir-9aSP} Avic\GFP^{EGFP.Scer\UAS-mir-276aSP} Avic\GFP^{EGFP.Scer\UAS-Scramble-SP} Disc\RFP^{mCherry.Scer\UAS.mir-9aSP} ena²¹⁰ ena^Scer\UAS.cCa mir-8^Scer\UAS.cLa mir-8^Δ2 mir-9a^E39 mir-9a^J22 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^Dll-md23 Scer\GAL4^elav.PLu Scer\GAL4^ey.PH Scer\GAL4^GMR.PF Scer\GAL4^how-24B Scer\GAL4^mir-8-NP5247 Scer\GAL4^ptc-559.1 Scer\GAL4^vg.PM Scer\GAL4^αTub84B.PL Zzzz\actA^{FP4mito.Scer\UAS.T:Avic\GFP-EGFP}
Constructs (18)
	P{GAL4-elav.L} P{GAL4-ey.H} P{GAL4-ninaE.GMR} P{GAL4-vg.M} P{GMR-yan^ACT} P{Tub-eGFP.mir-8} P{Tub-eGFP.nerfin-1.3'UTR} P{tubP-GAL4} P{UAS-eGFP.mir-7SP} P{UAS-eGFP.mir-8SP} P{UAS-eGFP.mir-9aSP} P{UAS-eGFP.mir-276aSP} P{UAS-eGFP.Scramble-SP} P{UAS-ena.C} P{UAS-GFP-FP4mito} P{UAS-mCD8::GFP.L} P{UAS-mCherry.mir9aSP} P{UAS-mir-8.L}
Genes (10)
	aop Avic\GFP Disc\RFP ena mir-7 mir-8 mir-9a Mmus\Cd8a Scer\GAL4 Zzzz\actA
Insertions (4)
	P{GawB}Dll^md23 P{GawB}how^24B P{GawB}NP5247 P{GawB}ptc^559.1
Natural transposons (1)
	P-element