Open Close
Reference
Citation
Witsell, A., Kane, D.P., Rubin, S., McVey, M. (2009). Removal of the bloom syndrome DNA helicase extends the utility of imprecise transposon excision for making null mutations in Drosophila.  Genetics 183(3): 1187--1193.
FlyBase ID
FBrf0209368
Publication Type
Research paper
Abstract

Transposable elements are frequently used in Drosophila melanogaster for imprecise excision screens to delete genes of interest. However, these screens are highly variable in the number and size of deletions that are recovered. Here, we show that conducting excision screens in mus309 mutant flies that lack DmBlm, the Drosophila ortholog of the Bloom syndrome protein, increases the percentage and overall size of flanking deletions recovered after excision of either P or Minos elements.

PubMed ID
PubMed Central ID
PMC2778970 (PMC) (EuropePMC)
Related Publication(s)
Note

Super-sized deletions: Improved transposon excision screens using a mus309 mutant background.
Witsell et al., 2010, Fly 4(2): 137--140 [FBrf0211090]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Genes (9)
    Natural transposons (4)