FlyBase Reference Report: Cook et al., 2009.11.25, Isolation and characterization of Df(3R)BSC848.

Reference

Citation

Cook, K., Christensen, S., Cook, K. (2009.11.25). Isolation and characterization of Df(3R)BSC848.

FlyBase ID

FBrf0209446

Publication Type

Personal communication to FlyBase

Abstract

PubMed ID

PubMed Central ID

Text of Personal Communication

Df(3R)BSC848 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG10420^f00587 and P{XP}d06042. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w¹¹¹⁸; P{hs-hid}3, Dr¹/TM6C, Sb¹ cu¹ females crossed to P{hsFLP}1, y¹ w¹¹¹⁸; PBac{WH}CG10420^f00587/P{XP}d06042 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC848 from the segment of PBac{WH}CG10420^f00587 to the left of its FRT site and the segment of P{XP}d06042 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3R)BSC848 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3R:21059545 ;21118634--21118719 and the cytological breakpoints predicted from these coordinates are 96C7;96D1. Df(3R)BSC848 failed to complement bam^Delta86.