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Yao, C.K., Lin, Y.Q., Ly, C.V., Ohyama, T., Haueter, C.M., Moiseenkova-Bell, V.Y., Wensel, T.G., Bellen, H.J. (2009). A synaptic vesicle-associated Ca2+ channel promotes endocytosis and couples exocytosis to endocytosis.  Cell 138(5): 947--960.
FlyBase ID
FBrf0209490
Publication Type
Research paper
Abstract

Synaptic vesicle (SV) exo- and endocytosis are tightly coupled to sustain neurotransmission in presynaptic terminals, and both are regulated by Ca(2+). Ca(2+) influx triggered by voltage-gated Ca(2+) channels is necessary for SV fusion. However, extracellular Ca(2+) has also been shown to be required for endocytosis. The intracellular Ca(2+) levels (<1 microM) that trigger endocytosis are typically much lower than those (>10 microM) needed to induce exocytosis, and endocytosis is inhibited when the Ca(2+) level exceeds 1 microM. Here, we identify and characterize a transmembrane protein associated with SVs that, upon SV fusion, localizes at periactive zones. Loss of Flower results in impaired intracellular resting Ca(2+) levels and impaired endocytosis. Flower multimerizes and is able to form a channel to control Ca(2+) influx. We propose that Flower functions as a Ca(2+) channel to regulate synaptic endocytosis and hence couples exo- with endocytosis.

PubMed ID
PubMed Central ID
PMC2749961 (PMC) (EuropePMC)
Related Publication(s)
Note

A new ion channel blooms at the synapse.
Kuo and Trussell, 2009, Neuron 63(5): 566--567 [FBrf0215063]

Flowers for synaptic endocytosis.
Brose and Neher, 2009, Cell 138(5): 836--837 [FBrf0208782]

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    Language of Publication
    English
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    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
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