Reference Report

Reference
Citation	Verstreken, P., Ohyama, T., Haueter, C., Habets, R.L.P., Lin, Y.Q., Swan, L.E., Ly, C.V., Venken, K.J.T., De Camilli, P., Bellen, H.J. (2009). Tweek, an evolutionarily conserved protein, is required for synaptic vesicle recycling. Neuron 63(2): 203--215. (Export to RIS)
FlyBase ID	FBrf0209491
Publication Type	Research paper
PubMed ID	19640479
PubMed Abstract	Synaptic vesicle endocytosis is critical for maintaining synaptic communication during intense stimulation. Here we describe Tweek, a conserved protein that is required for synaptic vesicle recycling. tweek mutants show reduced FM1-43 uptake, cannot maintain release during intense stimulation, and harbor larger than normal synaptic vesicles, implicating it in vesicle recycling at the synapse. Interestingly, the levels of a fluorescent PI(4,5)P(2) reporter are reduced at tweek mutant synapses, and the probe is aberrantly localized during stimulation. In addition, various endocytic adaptors known to bind PI(4,5)P(2) are mislocalized and the defects in FM1-43 dye uptake and adaptor localization are partially suppressed by removing one copy of the phosphoinositide phosphatase synaptojanin, suggesting a role for Tweek in maintaining proper phosphoinositide levels at synapses. Our data implicate Tweek in regulating synaptic vesicle recycling via an action mediated at least in part by the regulation of PI(4,5)P(2) levels or availability at the synapse.
DOI	10.1016/j.neuron.2009.06.017
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Aberrations (2)
	Df(2L)Exel8036 Df(2L)EY02585^Δ1
Alleles (17)
	ApepP^EY02585-Δ1 Avic\GFP^Scer\UAS.cUa Avic\GFP^{Scer\UAS.T:Hsap\MYC,T:Mmus\2xFYVE} CG42556^GD3470 Hsap\PLCD1^{mut.PH.Scer\UAS.T:Avic\GFP-EGFP} Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^elav-C155 Scer\GAL4^n-syb.PS synj¹ T:Avic\GFP^EGFP tweek¹ tweek² tweek^+tHB69 tweek^c01084 tweek^EY02585 tweek^EY02585-Δ1 tweek^GD8917
Constructs (8)
	P{GD3470} P{GD8917} P{n-syb-GAL4.S} P{tweek^+tHB69} P{UAS-GFP.U} P{UAS-GFP-myc-2xFYVE} P{UAS-PLCδ-PH-EGFP} P{UAS-PLCδ-PHmut-EGFP}
Genes (14)
	AP-2α ApepP Avic\GFP CG42556 Dap160 endoA GluRIIC Hsap\PLCD1 lap Scer\GAL4 shi stnB synj tweek
Insertions (5)
	P{EPgy2}ApepP^EY02585 P{EPgy2}CG31739^EY12630 P{GawB}elav^C155 P{SUPor-P}KG05250 PBac{PB}tweek^c01084
Natural transposons (1)
	P-element