Reference Report

Reference
Citation	Steinhauer, J., Gijón, M.A., Riekhof, W.R., Voelker, D.R., Murphy, R.C., Treisman, J.E. (2009). Drosophila Lysophospholipid Acyltransferases Are Specifically Required for Germ Cell Development. Mol. Biol. Cell 20(24): 5224--5235. (Export to RIS)
FlyBase ID	FBrf0209500
Publication Type	Research paper
PubMed ID	19864461
PubMed Abstract	Enzymes of the membrane-bound O-acyltransferase (MBOAT) family add fatty acyl chains to a diverse range of protein and lipid substrates. A chromosomal translocation disrupting human MBOAT1 results in a novel syndrome characterized by male sterility and brachydactyly. We have found that the Drosophila homologues of MBOAT1, Oysgedart (Oys), Nessy (Nes), and Farjavit (Frj), are lysophospholipid acyltransferases. When expressed in yeast, these MBOATs esterify specific lysophospholipids preferentially with unsaturated fatty acids. Generating null mutations for each gene allowed us to identify redundant functions for Oys and Nes in two distinct aspects of Drosophila germ cell development. Embryos lacking both oys and nes show defects in the ability of germ cells to migrate into the mesoderm, a process guided by lipid signals. In addition, oys nes double mutant adult males are sterile due to specific defects in spermatid individualization. oys nes mutant testes, as well as single, double, and triple mutant whole adult animals, show an increase in the saturated fatty acid content of several phospholipid species. Our findings suggest that lysophospholipid acyltransferase activity is essential for germline development and could provide a mechanistic explanation for the etiology of the human MBOAT1 mutation.
DOI	10.1091/mbc.E09-05-0382
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Aberrations (2)
	Df(2R)X3 Df(3R)crb87-5
Alleles (18)
	frj^EY06644 frj^Δ11 frj^Δ30 Hmgcr^{Scer\UAS.cvDa} jar³²² nes^EY22898 nes^{Scer\UAS.T:Avic\GFP} nes^Δ17 nes^Δ52 nes^Δ80 oys^KG00817 oys^{Scer\UAS.T:Avic\GFP} oys^Δ67 Scer\GAL4^c564 Scer\GAL4^elav-C155 Scer\GAL4^ptc-559.1 Scer\GAL4^tub.PU wun2^{αTub84B.T:Hsap\MYC}
Constructs (7)
	P{EPgy2} P{SUPor-P} P{tub-GAL4.U} P{UAS-Hmgcr.vD} P{UAS-nes.GFP} P{UAS-oys.GFP} P{αTub84B(-FRT)wun2.myc}
Genes (15)
	CG8112 CG34220 frj Hmgcr jar mdy nes Nrt oys por Prosap rasp Scer\GAL4 T:Avic\GFP wun2
Insertions (6)
	P{EPgy2}frj^EY06644 P{EPgy2}nes^EY22898 P{GawB}c564 P{GawB}elav^C155 P{GawB}ptc^559.1 P{SUPor-P}oys^KG00817
Natural transposons (1)
	P-element