Reference Report

Reference
Citation	Graf, E.R., Daniels, R.W., Burgess, R.W., Schwarz, T.L., DiAntonio, A. (2009). Rab3 Dynamically Controls Protein Composition at Active Zones. Neuron 64(5): 663--677. (Export to RIS)
FlyBase ID	FBrf0209504
Publication Type	Research paper
PubMed ID	20005823
PubMed Abstract	Synaptic transmission requires the localization of presynaptic release machinery to active zones. Mechanisms regulating the abundance of such synaptic proteins at individual release sites are likely determinants of site-specific synaptic efficacy. We now identify a role for the small GTPase Rab3 in regulating the distribution of presynaptic components to active zones. At Drosophila rab3 mutant NMJs, the presynaptic protein Bruchpilot, calcium channels, and electron-dense T bars are concentrated at a fraction of available active zones, leaving the majority of sites devoid of these key presynaptic release components. Late addition of Rab3 to mutant NMJs rapidly reverses this phenotype by recruiting Brp to sites previously lacking the protein, demonstrating that Rab3 can dynamically control the composition of the presynaptic release machinery. While previous studies of Rab3 have focused on its role in the synaptic vesicle cycle, these findings demonstrate an additional and unexpected function for Rab3 in the localization of presynaptic proteins to active zones.
DOI	10.1016/j.neuron.2009.11.002
Related Publication(s)
Personal communication to FlyBase	Location data for the Rab3[rup] deletion. Graf E. and DiAntonio A., 2012.12.18, Location data for the Rab3[rup] deletion. [FBrf0220460]
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Aberrations (1)
	Df(2R)ED2076
Alleles (12)
	brp^{dsRNA.C.Scer\UAS} brp^Scer\UAS.cWa cac^{Scer\UAS.T:Avic\GFP-EGFP} GluRIIC^{dsRNA.Scer\UAS} Rab3^{N134I.Scer\UAS} Rab3^rup Rab3^Scer\UAS.cGa Scer\GAL4^elav.PLu Scer\GAL4^{elav.Switch.PO} Scer\GAL4^how-24B Scer\GAL4^VGlut.PD w^+mC
Constructs (9)
	P{elav-Switch.O} P{GAL4-elav.L} P{UAS-brp.dsRNA.C} P{UAS-brp.W} P{UAS-cac1-EGFP} P{UAS-GluRIIC.RNAi} P{UAS-Rab3.G} P{UAS-Rab3.N134I} P{VGlut-GAL4.D}
Genes (8)
	brp cac GluRIIC Pak Rab3 Scer\GAL4 VGlut w
Insertions (1)
	P{GawB}how^24B
Natural transposons (1)
	P-element
Transcripts (1)
	Scer\GAL4^VGlut.PDRA