Reference Report

Reference
Citation	Shafer, O.T., Taghert, P.H. (2009). RNA-Interference Knockdown of Drosophila Pigment Dispersing Factor in Neuronal Subsets: The Anatomical Basis of a Neuropeptide's Circadian Functions. PLoS ONE 4(12): e8298. (Export to RIS)
FlyBase ID	FBrf0209505
Publication Type	Research paper
PubMed ID	20011537
PubMed Abstract	In animals, neuropeptide signaling is an important component of circadian timekeeping. The neuropeptide pigment dispersing factor (PDF) is required for several aspects of circadian activity rhythms in Drosophila.Here we investigate the anatomical basis for PDF's various circadian functions by targeted PDF RNA-interference in specific classes of Drosophila neuron. We demonstrate that PDF is required in the ventro-lateral neurons (vLNs) of the central brain and not in the abdominal ganglion for normal activity rhythms. Differential knockdown of PDF in the large or small vLNs indicates that PDF from the small vLNs is likely responsible for the maintenance of free-running activity rhythms and that PDF is not required in the large vLNs for normal behavior. PDF's role in setting the period of free-running activity rhythms and the proper timing of evening activity under light:dark cycles emanates from both subtypes of vLN, since PDF in either class of vLN was sufficient for these aspects of behavior.These results reveal the neuroanatomical basis PDF's various circadian functions and refine our understanding of the clock neuron circuitry of Drosophila.
DOI	10.1371/journal.pone.0008298
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Alleles (9)
	Dcr-2^Scer\UAS.cDa Ecol\lacZ^{Scer\UAS.T:SV40\nls2} Pdf⁰¹ Pdf^GD299 Scer\GAL4^dimm-929 Scer\GAL4^Dot.PK Scer\GAL4^Mai179 Scer\GAL4^R6 Scer\GAL4^tim.Scer\UAS
Constructs (5)
	P{Dot-GAL4.K} P{GD299} P{UAS-Dcr-2.D} P{UAS-lacZ.NZ} P{UAS-tim-GAL4}
Genes (12)
	crc Dcr-2 dimm Dot Ecol\lacZ elav l(2)Mai179 odd Pdf per Scer\GAL4 tim
Insertions (3)
	P{?GawB}crc^R6 P{GawB}dimm⁹²⁹ P{GawB}l(2)Mai179^Mai179
Transcripts (5)
	Scer\GAL4^dimm-929RA Scer\GAL4^Dot.PKRA Scer\GAL4^Mai179RA Scer\GAL4^R6RA Scer\GAL4^tim.Scer\UASRA