Reference Report

Reference
Citation	Koike-Kumagai, M., Yasunaga, K.I., Morikawa, R., Kanamori, T., Emoto, K. (2009). The target of rapamycin complex 2 controls dendritic tiling of Drosophila sensory neurons through the Tricornered kinase signalling pathway. EMBO J. 28(24): 3879--3892. (Export to RIS)
FlyBase ID	FBrf0209531
Publication Type	Research paper
PubMed ID	19875983
PubMed Abstract	To cover the receptive field completely and non-redundantly, neurons of certain functional groups arrange tiling of their dendrites. In Drosophila class IV dendrite arborization (da) neurons, the NDR family kinase Tricornered (Trc) is required for homotypic repulsion of dendrites that facilitates dendritic tiling. We here report that Sin1, Rictor, and target of rapamycin (TOR), components of the TOR complex 2 (TORC2), are required for dendritic tiling of class IV da neurons. Similar to trc mutants, dendrites of sin1 and rictor mutants show inappropriate overlap of the dendritic fields. TORC2 components physically and genetically interact with Trc, consistent with a shared role in regulating dendritic tiling. Moreover, TORC2 is essential for Trc phosphorylation on a residue that is critical for Trc activity in vivo and in vitro. Remarkably, neuronal expression of a dominant active form of Trc rescues the tiling defects in sin1 and rictor mutants. These findings suggest that TORC2 likely acts together with the Trc signalling pathway to regulate the dendritic tiling of class IV da neurons, and thus uncover the first neuronal function of TORC2 in vivo.
DOI	10.1038/emboj.2009.312
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Aberrations (1)
	Df(2R)BSC11
Alleles (17)
	Akt1¹ Avic\GFP^1.E.ppk rictor^Scer\UAS.cHa rictor^Δ2 S6k^l-1 Scer\GAL4^109(2)80 Scer\GAL4^elav-C155 Scer\GAL4^ppk.PG Scer\GAL4^unspecified Sin1^e03756 Sin1^{Scer\UAS.T:Zzzz\FLAG} Tor^ΔP trc¹ trc^Scer\UAS.cUa trc^{Scer\UAS.T:Myr-Src64B} trc^{T449A.Scer\UAS.T:Myr-Src64B} w^+mC
Constructs (7)
	P{ppk-EGFP.1} P{ppk-GAL4.G} P{UAS-rictor.H} P{UAS-Sin1.Flag} P{UAS-trc.Myr.T449A} P{UAS-trc.Myr} P{UAS-trc.U}
Genes (13)
	Akt1 Avic\GFP hpo rictor S6k Scer\GAL4 Sin1 T:Myr-Src64B T:Zzzz\FLAG Tor trc w wts
Insertions (3)
	P{GawB}109(2)80 P{GawB}elav^C155 PBac{RB}Sin1^e03756
Natural transposons (1)
	P-element