Reference Report

Reference
Citation	Weiss, A., Charbonnier, E., Ellertsdóttir, E., Tsirigos, A., Wolf, C., Schuh, R., Pyrowolakis, G., Affolter, M. (2010). A conserved activation element in BMP signaling during Drosophila development. Nat. Struct. Mol. Biol. 17(1): 69--76. (Export to RIS)
FlyBase ID	FBrf0209559
Publication Type	Research paper
PubMed ID	20010841
PubMed Abstract	The transforming growth factor beta (TGF-beta) family member Decapentaplegic (Dpp) is a key regulator of patterning and growth in Drosophila development. Previous studies have identified a short DNA motif called the silencer element (SE), which recruits a trimeric Smad complex and the repressor Schnurri to downregulate target enhancers upon Dpp signaling. We have now isolated the minimal enhancer of the dad gene and discovered a short motif we termed the activating element (AE). The AE is similar to the SE and recruits the Smad proteins via a conserved mechanism. However, the AE and SE differ at important nucleotide positions. As a consequence, the AE does not recruit Schnurri but rather integrates repressive input by the default repressor Brinker and activating input by the Smad signal transducers Mothers against Dpp (Mad) and Medea via competitive DNA binding. The AE allows the identification of hitherto unknown direct Dpp targets and is functionally conserved in vertebrates.
DOI	10.1038/nsmb.1715
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Struct. Mol. Biol.
Title	Nature Structural and Molecular Biology
Publication Year	2004-
ISBN/ISSN	1545-9993 1545-9985
Data from Reference
Alleles (28)
	brk^M68 brk^Scer\UAS.cJa brk^{Scer\UAS.T:Hsim\VP16} Ecol\lacZ^Dad.5xAE-1 Ecol\lacZ^Dad.5xAE Ecol\lacZ^Dad.5xHASE Ecol\lacZ^Dad.5xNASE Ecol\lacZ^Dad.5xSE+1 Ecol\lacZ^Dad.5xSE Ecol\lacZ^Dad.13 Ecol\lacZ^Dad.13PO Ecol\lacZ^Dad.13V Ecol\lacZ^Dad.AEd Ecol\lacZ^Dad.Int52 Ecol\lacZ^Dad.m1m4 Ecol\lacZ^Dad.NASE Ecol\lacZ^Dad.SBEd Ecol\lacZ^kni.AE19 Ecol\lacZ^kni.AE20 Ecol\lacZ^kni.AE20BtM Ecol\lacZ^kni.AE20d Ecol\lacZ^kni.AE20NASE Mad¹² Scer\GAL4^prd.RG1 shn^{Scer\UAS.T:Hsim\VP16} tkv⁸ tkv^{Q253D.Scer\FRT.Scer\UAS} tkv^{Q253D.Scer\UAS.cNc}
Constructs (25)
	P{Dad-lacZ.5xAE-1} P{Dad-lacZ.5xAE} P{Dad-lacZ.5xHASE} P{Dad-lacZ.5xNASE} P{Dad-lacZ.5xSE} P{Dad-lacZ.5xSE+1} P{Dad-lacZ.13} P{Dad-lacZ.13PO} P{Dad-lacZ.13V} P{Dad-lacZ.AEd} P{Dad-lacZ.Int52} P{Dad-lacZ.m1m4} P{Dad-lacZ.NASE} P{Dad-lacZ.SBEd} P{GAL4-prd.F} P{kni-lacZ.AE19} P{kni-lacZ.AE20} P{kni-lacZ.AE20BtM} P{kni-lacZ.AE20d} P{kni-lacZ.AE20NASE} P{UAS(FRT.y⁺CD2)tkv.Q253D.Nc} P{UAS(-FRT)tkv.Q253D.Nc} P{UAS-brk.J} P{UAS-brk.VP16} P{UAS-shn.VP16}
Genes (20)
	brk cv-2 Dad Doc1 Doc2 Doc3 dpp Ecol\lacZ elB kni knrl Mad Med pnr Scer\GAL4 shn T:Hsim\VP16 tgo tkv trh
Natural transposons (1)
	P-element
Sequence features (11)
	cv-2_ae6 Dad_dad13 dad_DadInt52 Doc2_ae7 Doc2_ae8 Doc2_ae9 Doc2_ae10 elB_ae12 kni_AE19 kni_AE20 pnr_ae21