Reference Report

Reference
Citation	Zheng, X., Mann, R.K., Sever, N., Beachy, P.A. (2010). Genetic and biochemical definition of the Hedgehog receptor. Genes Dev. 24(1): 57--71. (Export to RIS)
FlyBase ID	FBrf0209607
Publication Type	Research paper
PubMed ID	20048000
PubMed Abstract	Although the transporter-like protein Patched (Ptc) is genetically implicated in reception of the extracellular Hedgehog (Hh) protein signal, a clear definition of the Hh receptor is complicated by the existence of additional Hh-binding proteins and, in Drosophila, by the lack of physical evidence for direct binding of Hh to Ptc. Here we show that activity of Ihog (Interference hedgehog), or of its close relative Boi (Brother of Ihog), is absolutely required for Hh biological response and for sequestration of the Hh protein to limit long-range signaling. We demonstrate that Ihog interacts directly with Ptc, is required for presentation of Ptc on the cell surface, and that Ihog and Ptc are both required for high-affinity Hh binding. On the basis of their joint roles in ligand binding, signal transduction, and receptor trafficking, we conclude that Ihog and Ptc together constitute the Drosophila Hh receptor.
DOI	10.1101/gad.1870310
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (15)
	boi^KO boi^Scer\UAS.cZa Ecol\lacZ^dpp.BS3.0 hh^Scer\UAS.cAa ihog^{1-742.Scer\UAS} ihog^GLC ihog^{K653E.Q655E.Scer\UAS} ihog^Scer\UAS.cZa ihog^{ΔFn1.Scer\UAS} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Pka-C1^B3 ptc^{1130X.Scer\UAS} ptc^Scer\UAS.cJa Scer\FLP1^hs.PG Scer\GAL4^Act5C.PI
Constructs (12)
	P{Act5C-GAL4} P{BS3.0} P{hsFLP} P{UAS-boi.Z} P{UAS-hh.A} P{UAS-ihog.Fn2} P{UAS-ihog.Z} P{UAS-ihog.ΔCTD} P{UAS-ihog.ΔFn1} P{UAS-mCD8::GFP.L} P{UAS-ptc.1130X} P{UAS-ptc.J}
Genes (15)
	boi ci dpp Ecol\lacZ en hh ihog Mmus\Cd8a Pka-C1 ptc Scer\FLP1 Scer\GAL4 smo wg z
Insertions (1)
	P{neoFRT}40A
Natural transposons (1)
	P-element