Reference Report

Reference
Citation	Venken, K.J.T., Bellen, H.J., Hoskins, R.A. (2009.12.19). MIMIC transposable element ("Minos Mediated Integration Casette") (Export to RIS)
FlyBase ID	FBrf0209655
Publication Type	Personal communication to FlyBase
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PubMed Abstract
Text of Personal Communication	This vector integrates often into introns (30%) and once MIMIC is inserted, one can replace the DNA between the two attP sites with whatever DNA using the Recombination Mediated Cassette Exchange (RMCE) methodology. This will allow us to tag genes, insert stop codons, modify loci etc. Sequence has been submitted to GenBank; annotated features: FEATURES Location/Qualifiers source 1..7267 /organism="Drosophila melanogaster" /mol_type="other DNA" /db_xref="taxon:7227" misc_feature 104..356 /note="Minos transposon inverted repeat" misc_feature 405..522 /note="phiC31 attP site" misc_feature 523..622 /note="Drosophila melanogaster MHC Splice acceptor site" CDS 641..1360 /note="Fluorescent molecule" /codon_start=1 /product="EGFPmut4" misc_feature 641..1360 /note="EGFP" misc_feature 1369..1610 /note="SV40 polyA signal" misc_feature 1623..6791 /note="Drosophila melanogaster yellow+ transgenesis marker" misc_feature complement(6792..6903) /note="phiC31 attP site" misc_feature 6949..7202 /note="Minos transposon inverted repeat"
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Language of Publication	English
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	Mi{MIC}