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Venken, K.J.T., Bellen, H.J., Hoskins, R.A. (2009.12.19). MIMIC transposable element ("Minos Mediated Integration Casette") 
FlyBase ID
FBrf0209655
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Personal communication to FlyBase
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This vector integrates often into introns (30%) and once MIMIC is inserted, one can replace the DNA between the two attP sites with whatever DNA using the Recombination Mediated Cassette Exchange (RMCE) methodology. This will allow us to tag genes, insert stop codons, modify loci etc.  Sequence has been submitted to GenBank; annotated features:
FEATURES             Location/Qualifiers
    source          1..7267
                    /organism="Drosophila melanogaster"
                    /mol_type="other DNA"
                    /db_xref=" taxon:7227 "
    misc_feature    104..356
                    /note="Minos transposon inverted repeat"
    misc_feature    405..522
                    /note="phiC31 attP site"
    misc_feature    523..622
                    /note="Drosophila melanogaster MHC Splice acceptor site"
    CDS             641..1360
                    /note="Fluorescent molecule"
                    /codon_start=1
                    /product="EGFPmut4"
    misc_feature    641..1360
                    /note="EGFP"
    misc_feature    1369..1610
                    /note="SV40 polyA signal"
    misc_feature    1623..6791
                    /note="Drosophila melanogaster yellow+ transgenesis
                    marker"
    misc_feature    complement(6792..6903)
                    /note="phiC31 attP site"
    misc_feature    6949..7202
                    /note="Minos transposon inverted repeat"
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    English
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    Transgenic Constructs (1)