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Citation
Kong, E.C., Allouche, L., Chapot, P.A., Vranizan, K., Moore, M.S., Heberlein, U., Wolf, F.W. (2010). Ethanol-regulated genes that contribute to ethanol sensitivity and rapid tolerance in Drosophila.  Alcoholism, Clin. Exp. Res. 34(2): 302--316.
FlyBase ID
FBrf0209720
Publication Type
Research paper
Abstract
Increased ethanol intake, a major predictor for the development of alcohol use disorders, is facilitated by the development of tolerance to both the aversive and pleasurable effects of the drug. The molecular mechanisms underlying ethanol tolerance development are complex and are not yet well understood.To identify genetic mechanisms that contribute to ethanol tolerance, we examined the time course of gene expression changes elicited by a single sedating dose of ethanol in Drosophila, and completed a behavioral survey of strains harboring mutations in ethanol-regulated genes.Enrichment for genes in metabolism, nucleic acid binding, olfaction, regulation of signal transduction, and stress suggests that these biological processes are coordinately affected by ethanol exposure. We also detected a coordinate up-regulation of genes in the Toll and Imd innate immunity signal transduction pathways. A multi-study comparison revealed a small set of genes showing similar regulation, including increased expression of 3 genes for serine biosynthesis. A survey of Drosophila strains harboring mutations in ethanol-regulated genes for ethanol sensitivity and tolerance phenotypes revealed roles for serine biosynthesis, olfaction, transcriptional regulation, immunity, and metabolism. Flies harboring deletions of the genes encoding the olfactory co-receptor Or83b or the sirtuin Sir2 showed marked changes in the development of ethanol tolerance.Our findings implicate novel roles for these genes in regulating ethanol behavioral responses.
PubMed ID
PubMed Central ID
PMC2903447 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Alcoholism, Clin. Exp. Res.
    Title
    Alcoholism, Clinical and Experimental Research
    Publication Year
    1977
    ISBN/ISSN
    0145-6008
    Data From Reference