Reference Report

Reference
Citation	Wang, Z.A., Kalderon, D. (2009). Cyclin E-dependent protein kinase activity regulates niche retention of Drosophila ovarian follicle stem cells. Proc. Natl. Acad. Sci. U.S.A. 106(51): 21701--21706. (Export to RIS)
FlyBase ID	FBrf0209757
Publication Type	Research paper
PubMed ID	19966222
PubMed Abstract	Whether stem cells have unique cell cycle machineries and how they integrate with niche interactions remains largely unknown. We identified a hypomorphic cyclin E allele WX that strongly impairs the maintenance of follicle stem cells (FSCs) in the Drosophila ovary but does not reduce follicle cell proliferation or germline stem cell maintenance. CycE(WX) protein can still bind to the cyclin-dependent kinase catalytic subunit Cdk2, but forms complexes with reduced protein kinase activity measured in vitro. By creating additional CycE variants with different degrees of kinase dysfunction and expressing these and CycE(WX) at different levels, we found that higher CycE-Cdk2 kinase activity is required for FSC maintenance than to support follicle cell proliferation. Surprisingly, cycE(WX) FSCs were lost from their niches rather than arresting proliferation. Furthermore, FSC function was substantially restored by expressing either excess DE-cadherin or excess E2F1/DP, the transcription factor normally activated by CycE-Cdk2 phosphorylation of retinoblastoma proteins. These results suggest that FSC maintenance through niche adhesion is regulated by inputs that normally control S phase entry, possibly as a quality control mechanism to ensure adequate stem cell proliferation. We speculate that a positive connection between central regulators of the cell cycle and niche retention may be a common feature of highly proliferative stem cells.
DOI	10.1073/pnas.0909272106
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (25)
	CycE^1F36 CycE² CycE^{5A.Scer\UAS.cWa} CycE^{7A.Scer\UAS.cWa} CycE¹⁰⁴²⁷ CycE^AR95 CycE^JP CycE^KG00239 CycE^{RA.Scer\UAS.cWa} CycE^Scer\UAS.cWa CycE^{WX.Scer\UAS.cWa} CycE^WX dap^{Scer\UAS.cdNa} dap^unspecified Dp^Scer\UAS.cDa E2f^{dsRNA.Scer\UAS.cDa} E2f^Scer\UAS.cNa Scer\GAL4^Act5C.PI Scer\GAL4^Act5C.PP Scer\GAL4^{Scer\FRT.Act5C} Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} Scer\GAL4^tub.PU Scer\GAL4^unspecified shg^Scer\UAS.cSa th^Scer\UAS.cHa
Constructs (16)
	P{Act5C-GAL4} P{AyGAL4} P{GAL4} P{GAL4-Act5C(FRT.CD2).P} P{GAL4-Act5C(-FRT).P} P{tub-GAL4.U} P{UAS-CycE.7A} P{UAS-CycE.RA} P{UAS-CycE.W} P{UAS-CycE.WX} P{UAS-dap.dN} P{UAS-DIAP1.H} P{UAS-Dp.D} P{UAS-E2f.dsRNA} P{UAS-E2f.N} P{UAS-shg.CAD}
Genes (12)
	cdc2c CG31739 CycE dap Dp E2f Fas3 His1 l(2)k14505 Scer\GAL4 shg th
Natural transposons (1)
	P-element