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Nègre, N., Brown, C.D., Shah, P.K., Kheradpour, P., Morrison, C.A., Henikoff, J.G., Feng, X., Ahmad, K., Russell, S., White, R.A., Stein, L., Henikoff, S., Kellis, M., White, K.P. (2010). A comprehensive map of insulator elements for the Drosophila genome.  PLoS Genet. 6(1): e1000814.
FlyBase ID
FBrf0209760
Publication Type
Research paper
Abstract

Insulators are DNA sequences that control the interactions among genomic regulatory elements and act as chromatin boundaries. A thorough understanding of their location and function is necessary to address the complexities of metazoan gene regulation. We studied by ChIP-chip the genome-wide binding sites of 6 insulator-associated proteins-dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined insulators. We find two major classes of insulators defined by dCTCF/CP190/BEAF-32 and Su(Hw), respectively. Distributional analyses of insulators revealed that particular sub-classes of insulator elements are excluded between cis-regulatory elements and their target promoters; divide differentially expressed, alternative, and divergent promoters; act as chromatin boundaries; are associated with chromosomal breakpoints among species; and are embedded within active chromatin domains. Together, these results provide a map demarcating the boundaries of gene regulatory units and a framework for understanding insulator function during the development and evolution of Drosophila.

PubMed ID
PubMed Central ID
PMC2797089 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Class I and Class II insulator elements.
Negre et al., 2010.2.22, Class I and Class II insulator elements. [FBrf0210089]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS Genet.
    Title
    PLoS Genetics
    Publication Year
    2005-
    ISBN/ISSN
    1553-7404 1553-7390
    Data From Reference