Reference Report

Reference
Citation	Pankotai, T., Ujfaludi, Z., Vámos, E., Suri, K., Boros, I.M. (2010). The dissociable RPB4 subunit of RNA Pol II has vital functions in Drosophila. Mol. Genet. Genomics 283(1): 89--97. (Export to RIS)
FlyBase ID	FBrf0209781
Publication Type	Research paper
PubMed ID	19921261
PubMed Abstract	RNA polymerase II (Pol II) is composed of a ten subunit core and a two subunit dissociable subcomplex comprising the fourth and seventh largest subunits, RPB4 and RPB7. The evolutionary highly conserved RPB4/7 heterodimer is positioned in the Pol II such that it can make contact with various factors involved in RNA biogenesis and is believed to play roles both during the process of transcription and post-transcription. A detailed analysis of RPB4/7 function in a multicellular eukaryote, however, is lacking partly because of the lack of a suitable genetic system. Here, we describe generation and initial analysis of Drosophila Rpb4 mutants. In the fly, RPB4 is a product of a bicistronic gene together with the ATAC histone acetyltransferase complex constituent ADA2a. DmAda2a and DmRpb4 are expressed during fly development at different levels. The structure of mature mRNA forms suggests that the production of DmADA2a and DmRPB4-specific mRNAs is ensured by alternative splicing. Genetic analysis indicates that both DmRPB4 and DmADA2a play essential roles, because their absence results in lethality in early and late larval stages, respectively. Upon stress of high temperature or nutritional starvation, the levels of RPB4 and ADA2a messages change differently. RPB4 colocalizes with Pol II to several sites on polytene chromosomes, however, at selected locus, the abundances of Pol II and RPB4 vary greatly. Our data suggest no tight functional link between DmADA2a and DmRPB4, and reveal differences in the abundances of Pol II core subunits and RPB4 localized at specific regions on polytene chromosomes, supporting the suggested role of RPB4 outside of transcription-engaged Pol II complexes.
DOI	10.1007/s00438-009-0499-6
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Genet. Genomics
Title	Molecular Genetics and Genomics
Publication Year	2001-
ISBN/ISSN	1617-4615
Data from Reference
Aberrations (1)
	Df(3R)d189
Alleles (10)
	Ada2a^+t7.1 Ada2a^Dtl-Ada2a Rpb4^+t7.1 Rpb4^NIG.31237R Rpb4^Scer\UAS.cPa RpII33^{Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^C147 Scer\GAL4^da.G32 tatl^+t7.1 tatl^Dtl-Ada2a
Constructs (6)
	P{CaSpeR4-Dtl⁺Ada2a⁺} P{CaSpeR4-Dtl⁺Ada2a⁺Rpb4⁺} P{GAL4-da.G32} P{NIG.31237R} P{UAS-.P} P{UAS-RpII33-EGFP}
Genes (11)
	Ada2a Hsp70Aa Hsp70Ba Hsp70Bb Hsp70Bbb Hsp70Bc Rpb4 RpII33 RpII215 Scer\GAL4 tatl
Insertions (1)
	P{GawB}C147
Natural transposons (1)
	P-element