Reference Report

Reference
Citation	Bejarano, F., Smibert, P., Lai, E.C. (2010). miR-9a prevents apoptosis during wing development by repressing Drosophila LIM-only. Dev. Biol. 338(1): 63--73. (Export to RIS)
FlyBase ID	FBrf0209786
Publication Type	Research paper
PubMed ID	19944676
PubMed Abstract	Loss of Drosophila mir-9a induces a subtle increase in sensory bristles, but a substantial loss of wing tissue. Here, we establish that the latter phenotype is largely due to ectopic apoptosis in the dorsal wing primordium, and we could rescue wing development in the absence of this microRNA by dorsal-specific inhibition of apoptosis. Such apoptosis was a consequence of de-repressing Drosophila LIM-only (dLMO), which encodes a transcriptional regulator of wing and neural development. We observed cell-autonomous elevation of endogenous dLMO and a GFP-dLMO 3'UTR sensor in mir-9a mutant wing clones, and heterozygosity for dLMO rescued the apoptosis and wing defects of mir-9a mutants. We also provide evidence that dLMO, in addition to senseless, contributes to the bristle defects of the mir-9a mutant. Unexpectedly, the upregulation of dLMO, loss of Cut, and adult wing margin defects seen with mir-9a mutant clones were not recapitulated by clonal loss of the miRNA biogenesis factors Dicer-1 or Pasha, even though these mutant conditions similarly de-repressed miR-9a and dLMO sensor transgenes. Therefore, the failure to observe a phenotype upon conditional knockout of a miRNA processing factor does not reliably indicate the lack of critical roles of miRNAs in a given setting.
DOI	10.1016/j.ydbio.2009.11.025
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (19)
	Avic\GFP^Bx.3'UTR.tub Avic\GFP^{mir-9a.3'UTR.tub} BacA\p35^Scer\UAS.cHa Bx¹ Bx^hdpR26 Bx^{Scer\UAS.T:Ivir\HA1} Dcr-1^Q1147X mir-9a^E39 mir-9a^J22 mir-9a^Scer\UAS.cLa mir-9a^{Scer\UAS.T:Disc\RFP-DsRed2} pasha^unspecified Scer\GAL4^ap-md544 Scer\GAL4^ptc-559.1 sens^E2 sens^Ly-1 sens^Scer\UAS.cNa T:Disc\RFP^DsRed2 th^Scer\UAS.cHa
Constructs (8)
	P{tub-GFP.Bx.3'UTR} P{tub-GFP.mir-9a.3'UTR} P{UAS-Bx.T:HA1} P{UAS-DIAP1.H} P{UAS-DsRed-mir-9a} P{UAS-mir-9a.L} P{UAS-p35.H} P{UAS-sens.N}
Genes (11)
	Avic\GFP BacA\p35 Bx ct Dcr-1 mir-9a pasha Scer\GAL4 sens th wg
Insertions (2)
	P{GawB}ap^md544 P{GawB}ptc^559.1
Natural transposons (1)
	P-element