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Hua, H., Georgiev, O., Schaffner, W., Steiger, D. (2010). Human copper transporter Ctr1 is functional in Drosophila, revealing a high degree of conservation between mammals and insects.  J. Biol. Inorg. Chem. 15(1): 107--113.
FlyBase ID
FBrf0209805
Publication Type
Research paper
Abstract

Living cells have to carefully control the intracellular concentration of trace metals, especially of copper, which is at the same time essential but owing to its redox activity can also facilitate generation of reactive oxygen species. Mammals have two related copper transporters, Ctr1 and Ctr2, with Ctr1 playing the major role. The fruit fly Drosophila has three family members, termed Ctr1A, Ctr1B, and Ctr1C. Ctr1A is expressed throughout development, and a null mutation causes lethality at an early stage. Ctr1B ensures efficient copper uptake in the intestinal tract, whereas Ctr1C is mainly expressed in male gonads. Ectopic expression of Ctr1 transporters in Drosophila causes toxic effects due to excessive copper uptake. Here, we compare the effects of human Ctr1 (hCtr1) with those of the Drosophila homologs Ctr1A and Ctr1B in two overexpression assays. Whereas the overexpression of Drosophila Ctr1A and Ctr1B results in strong phenotypes, expression of hCtr1 causes only a very mild phenotype, indicating a low copper-import efficiency in the Drosophila system. However, this can be boosted by coexpressing the human copper chaperone CCS. Surprisingly, hCtr1 complements a lethal Ctr1A mutation at least as well as Ctr1A and Ctr1B transgenes. These findings reveal a high level of conservation between the mammalian and insect Ctr1-type copper importers, and they also demonstrate that the Drosophila Ctr1 proteins are functionally interchangeable.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Biol. Inorg. Chem.
    Title
    Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
    ISBN/ISSN
    0949-8257 1432-1327
    Data From Reference
    Alleles (9)
    Gene Groups (1)
    Genes (6)
    Human Disease Models (1)
    Natural transposons (2)
    Insertions (4)
    Experimental Tools (2)
    Transgenic Constructs (8)