Reference Report

Reference
Citation	Agrawal, N., Venkiteswaran, G., Sadaf, S., Padmanabhan, N., Banerjee, S., Hasan, G. (2010). Inositol 1,4,5-trisphosphate receptor and dSTIM function in Drosophila insulin-producing neurons regulates systemic intracellular calcium homeostasis and flight. J. Neurosci. 30(4): 1301--1313. (Export to RIS)
FlyBase ID	FBrf0209847
Publication Type	Research paper
PubMed ID	20107057
PubMed Abstract	Calcium (Ca(2+)) signaling is known to regulate the development, maintenance and modulation of activity in neuronal circuits that underlie organismal behavior. In Drosophila, intracellular Ca(2+) signaling by the inositol 1,4,5-trisphosphate receptor and the store-operated channel (dOrai) regulates the formation and function of neuronal circuits that control flight. Here, we show that restoring InsP(3)R activity in insulin-producing neurons of flightless InsP(3)R mutants (itpr) during pupal development can rescue systemic flight ability. Expression of the store operated Ca(2+) entry (SOCE) regulator dSTIM in insulin-producing neurons also suppresses compromised flight ability of InsP(3)R mutants suggesting that SOCE can compensate for impaired InsP(3)R function. Despite restricted expression of wild-type InsP(3)R and dSTIM in insulin-producing neurons, a global restoration of SOCE and store Ca(2+) is observed in primary neuronal cultures from the itpr mutant. These results suggest that restoring InsP(3)R-mediated Ca(2+) release and SOCE in a limited subset of neuromodulatory cells can influence systemic behaviors such as flight by regulating intracellular Ca(2+) homeostasis in a large population of neurons through a non-cell-autonomous mechanism.
DOI	10.1523/JNEUROSCI.3668-09.2010
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Alleles (21)
	Dcr-2^Scer\UAS.cDa Itp-r83A^ka1091 Itp-r83A^NIG.1063R Itp-r83A^Scer\UAS.cVa Itp-r83A^ug3 Itp-r83A^wc703 mAcR-60C^Scer\UAS.cVa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} olf186-F^GD3554 olf186-F^Scer\UAS.cZa Scer\GAL4^386Y Scer\GAL4^Akh.PG Scer\GAL4^Ccap.PP Scer\GAL4^Ddc.PL Scer\GAL4^dimm-929 Scer\GAL4^elav.PLu Scer\GAL4^elav-C155 Scer\GAL4^Ilp2.PR Scer\GAL80^ts.αTub84B Stim^GD16187 Stim^Scer\UAS.cAa
Constructs (15)
	P{Akh-gal4.G} P{Ccap-GAL4.P} P{Ddc-GAL4.L} P{GAL4-elav.L} P{GD3554} P{GD16187} P{Ilp2-GAL4.R} P{NIG.1063R} P{tubP-GAL80^ts} P{UAS-Dcr-2.D} P{UAS-Itp-r83A.V} P{UAS-mAChR.V} P{UAS-mCD8::GFP.L} P{UAS-Orai.Z} P{UAS-Stim.A}
Genes (10)
	Dcr-2 Ddc Ilp2 Itp-r83A mAcR-60C Mmus\Cd8a olf186-F Scer\GAL4 Scer\GAL80 Stim
Insertions (3)
	P{GawB}386Y P{GawB}dimm⁹²⁹ P{GawB}elav^C155
Transcripts (2)
	Scer\GAL4^Ddc.PLRA Scer\GAL4^Ilp2.PRRA