Reference Report

Reference
Citation	Stevens, N.R., Dobbelaere, J., Brunk, K., Franz, A., Raff, J.W. (2010). Drosophila Ana2 is a conserved centriole duplication factor. J. Cell Biol. 188(3): 313--323. (Export to RIS)
FlyBase ID	FBrf0209887
Publication Type	Research paper
PubMed ID	20123993
PubMed Abstract	In Caenorhabditis elegans, five proteins are required for centriole duplication: SPD-2, ZYG-1, SAS-5, SAS-6, and SAS-4. Functional orthologues of all but SAS-5 have been found in other species. In Drosophila melanogaster and humans, Sak/Plk4, DSas-6/hSas-6, and DSas-4/CPAP-orthologues of ZYG-1, SAS-6, and SAS-4, respectively-are required for centriole duplication. Strikingly, all three fly proteins can induce the de novo formation of centriole-like structures when overexpressed in unfertilized eggs. Here, we find that of eight candidate duplication factors identified in cultured fly cells, only two, Ana2 and Asterless (Asl), share this ability. Asl is now known to be essential for centriole duplication in flies, but no equivalent protein has been found in worms. We show that Ana2 is the likely functional orthologue of SAS-5 and that it is also related to the vertebrate STIL/SIL protein family that has been linked to microcephaly in humans. We propose that members of the SAS-5/Ana2/STIL family of proteins are key conserved components of the centriole duplication machinery.
DOI	10.1083/jcb.200910016
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Alleles (16)
	ana1^{Scer\UAS.P\T.T:Avic\GFP} ana2^{Scer\UAS.P\T.T:Avic\GFP} ana2^{Ubi.C.T:Avic\GFP} ana2^{Ubi.N.T:Avic\GFP} ana3^{Scer\UAS.P\T.T:Avic\GFP} asl^{Scer\UAS.P\T.T:Avic\GFP} asl^{Ubi.S.T:Avic\GFP} Cep97^{Scer\UAS.P\T.T:Avic\GFP} Cep135^{Scer\UAS.P\T.T:Avic\GFP} Cp110^{Scer\UAS.P\T.T:Avic\GFP} cp309^{PACT.Ubi.T:Disc\RFP-mRFP} Rcd4^{Scer\UAS.P\T.T:Avic\GFP} SAK^{Ubi.P.T:Avic\GFP} Sas-4^{Ubi.P.T:Avic\GFP} sas-6^{Ubi.P.T:Avic\GFP} Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}
Constructs (16)
	P{matα4-GAL-VP16} P{UASp-ana2-GFP} P{UASp-ana3-GFP} P{UASp-asl-GFP} P{UASp-Cp110-GFP} P{UASp-GFP-ana1} P{UASp-GFP-Cep97} P{UASp-GFP-Cep135} P{UASp-GFP-Rcd4} P{Ubi-ana2-GFP} P{Ubi-asl-GFP.S} P{Ubi-GFP-ana2} P{Ubi-GFP-Sak.P} P{Ubi-GFP-sas-6.P} P{Ubi-mRFP-PACT} P{Ubi-Sas-4-GFP.P}
Genes (14)
	ana1 ana2 ana3 asl Cep97 Cep135 Cp110 cp309 Rcd4 SAK Sas-4 sas-6 Scer\GAL4 T:Avic\GFP
Natural transposons (1)
	P-element