Reference Report

Reference
Citation	Spitzweck, B., Brankatschk, M., Dickson, B.J. (2010). Distinct Protein Domains and Expression Patterns Confer Divergent Axon Guidance Functions for Drosophila Robo Receptors. Cell 140(3): 409--420. (Export to RIS)
FlyBase ID	FBrf0209892
Publication Type	Research paper
PubMed ID	20144763
PubMed Abstract	The orthogonal array of axon pathways in the Drosophila CNS is constructed in part under the control of three Robo family axon guidance receptors: Robo1, Robo2 and Robo3. Each of these receptors is responsible for a distinct set of guidance decisions. To determine the molecular basis for these functional specializations, we used homologous recombination to create a series of 9 "robo swap" alleles: expressing each of the three Robo receptors from each of the three robo loci. We demonstrate that the lateral positioning of longitudinal axon pathways relies primarily on differences in gene regulation, not distinct combinations of Robo proteins as previously thought. In contrast, specific features of the Robo1 and Robo2 proteins contribute to their distinct functions in commissure formation. These specializations allow Robo1 to prevent crossing and Robo2 to promote crossing. These data demonstrate how diversification of expression and structure within a single family of guidance receptors can shape complex patterns of neuronal wiring.
DOI	10.1016/j.cell.2010.01.002
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Aberrations (1)
	Df(1)NetAB^Δ
Alleles (53)
	fra^23H9 fra^GA957 lea^KO.robo1-2 lea^KO.robo2-1 lea^KO.robo3 lea^KO.robo lea^{lea.HR.T:Ivir\HA1} lea^{lea.Scer\SceI.RS} lea^robo2-4 lea^robo2-8 lea^{robo3.HR.T:Ivir\HA1} lea^{robo3.Scer\SceI.RS} lea^{robo.HR.T:Ivir\HA1} lea^{robo.Scer\SceI.RS} lea^{robo.T:Ivir\HA1} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} robo3¹ robo3^KO.lea robo3^KO.robo robo3^{lea.HR.T:Ivir\HA1} robo3^{lea.Scer\SceI.RS} robo3^{robo3.HR.T:Ivir\HA1} robo3^{robo3.Scer\SceI.RS} robo3^{robo.HR.T:Ivir\HA1} robo3^{robo.Scer\SceI.RS} robo3^{robo.T:Ivir\HA1} robo::lea^{1-2.lea.HR.T:Ivir\HA1} robo::lea^{1-2.lea.Scer\SceI.RS} robo::lea^{2-1.lea.HR.T:Ivir\HA1} robo::lea^{2-1.lea.Scer\SceI.RS} robo::robo3^{1.CC0.3.CC1.robo.T:Ivir\HA1} robo::robo3^{1.CC1.3.CC2.robo.T:Ivir\HA1} robo::robo3^{1.CC2.3.CC3.robo.T:Ivir\HA1} robo::robo3^{1.Ig2.3.Ig3.robo.T:Ivir\HA1} robo::robo3^{1.TM.3.CC0.robo.T:Ivir\HA1} robo::robo3^{3.CC0.1.CC1.robo.T:Ivir\HA1} robo::robo3^{3.CC1.1.CC2.robo.T:Ivir\HA1} robo::robo3^{3.CC2.1.CC3.robo.T:Ivir\HA1} robo::robo3^{3.Ig2.1.Ig3.robo.T:Ivir\HA1} robo::robo3^{3.TM.1.CC0.robo.T:Ivir\HA1} robo¹ robo⁸ robo^KO.lea robo^KO.robo3 robo^{lea.HR.T:Ivir\HA1} robo^{lea.Scer\SceI.RS} robo^{robo3.HR.T:Ivir\HA1} robo^{robo3.Scer\SceI.RS} robo^{robo.HR.T:Ivir\HA1} robo^{robo.Scer\SceI.RS} robo^{robo.T:Ivir\HA1} robo^unspecified Scer\GAL4^Sema-2b.PS
Constructs (27)
	P{attP,y⁺,w^3'} P{FRT(robo1^robo1.Scer\SceI.RS)} P{FRT(robo1^robo2.Scer\SceI.RS)} P{FRT(robo1^robo3.Scer\SceI.RS)} P{FRT(robo2^robo1-2.Scer\SceI.RS)} P{FRT(robo2^robo1.Scer\SceI.RS)} P{FRT(robo2^robo2-1.Scer\SceI.RS)} P{FRT(robo2^robo2.Scer\SceI.RS)} P{FRT(robo2^robo3.Scer\SceI.RS)} P{FRT(robo3^robo1.Scer\SceI.RS)} P{FRT(robo3^robo2.Scer\SceI.RS)} P{FRT(robo3^robo3.Scer\SceI.RS)} P{robo1-robo1^CC0::robo3^CC1-HA} P{robo1-robo1^CC1::robo3^CC2-HA} P{robo1-robo1^CC2::robo3^CC3-HA} P{robo1-robo1^Ig2::robo3^Ig3-HA} P{robo1-robo1^TM::robo3^CC0-HA} P{robo1-robo1-HA} P{robo1-robo2-HA} P{robo1-robo3^CC0::robo1^CC1-HA} P{robo1-robo3^CC1::robo1^CC2-HA} P{robo1-robo3^CC2::robo1^CC3-HA} P{robo1-robo3^Ig2::robo1^Ig3-HA} P{robo1-robo3^TM::robo1^CC0-HA} P{robo1-robo3-HA} P{Sema-2b-GAL4.S} P{UAS-mCD8::GFP.L}
Genes (13)
	Fas2 fra lea Mmus\Cd8a NetA NetB robo robo3 robo::lea robo::robo3 Scer\GAL4 Sema-2b T:Ivir\HA1
Insertions (14)
	P{attP,y⁺,w^3'}VIE-274B P{robo1-robo1^CC0::robo3^CC1-HA}VIE-274B P{robo1-robo1^CC1::robo3^CC2-HA}VIE-274B P{robo1-robo1^CC2::robo3^CC3-HA}VIE-274B P{robo1-robo1^Ig2::robo3^Ig3-HA}VIE-274B P{robo1-robo1^TM::robo3^CC0-HA}VIE-274B P{robo1-robo1-HA}VIE-274B P{robo1-robo2-HA}VIE-274B P{robo1-robo3^CC0::robo1^CC1-HA}VIE-274B P{robo1-robo3^CC1::robo1^CC2-HA}VIE-274B P{robo1-robo3^CC2::robo1^CC3-HA}VIE-274B P{robo1-robo3^Ig2::robo1^Ig3-HA}VIE-274B P{robo1-robo3^TM::robo1^CC0-HA}VIE-274B P{robo1-robo3-HA}VIE-274B
Natural transposons (1)
	P-element