Reference Report

Reference
Citation	Gilsohn, E., Volk, T. (2010). Slowdown promotes muscle integrity by modulating integrin-mediated adhesion at the myotendinous junction. Development 137(5): 785--794. (Export to RIS)
FlyBase ID	FBrf0209925
Publication Type	Research paper
PubMed ID	20110313
PubMed Abstract	The correct assembly of the myotendinous junction (MTJ) is crucial for proper muscle function. In Drosophila, this junction comprises hemi-adherens junctions that are formed upon arrival of muscles at their corresponding tendon cells. The MTJ mainly comprises muscle-specific alphaPS2betaPS integrin receptors and their tendon-derived extracellular matrix ligand Thrombospondin (Tsp). We report the identification and functional analysis of a novel tendon-derived secreted protein named Slowdown (Slow). Homozygous slow mutant larvae exhibit muscle or tendon rupture, sluggish larval movement, partial lethality, and the surviving adult flies are unable to fly. These defects result from improper assembly of the embryonic MTJ. In slow mutants, Tsp prematurely accumulates at muscle ends, the morphology of the muscle leading edge changes and the MTJ architecture is aberrant. Slow was found to form a protein complex with Tsp. This complex is biologically active and capable of altering the morphology and directionality of muscle ends. Our analysis implicates Slow as an essential component of the MTJ, crucial for ensuring muscle and tendon integrity during larval locomotion.
DOI	10.1242/dev.043703
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (18)
	Avic\GFP^{S65T.Mhc.T:Btau\MAPT} if^k27e if^{Scer\UAS.cMBa} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Moe^{GMA.Scer\UAS.T:Avic\GFP-S65T} mys^din.Scer\UAS Scer\GAL4^e22c Scer\GAL4^Mef2.PR Scer\GAL4^repo Scer\GAL4^sr-md710 Scer\GAL4^tey-5053A slow¹ slow^{Scer\UAS.T:Ivir\HA1} sr^A.Scer\UAS Tsp^8R Tsp^{Scer\UAS.AD.cSa} Tsp^{Scer\UAS.AD.T:Avic\GFP} Tsp^{Scer\UAS.NTD.T:Avic\GFP}
Constructs (13)
	P{GAL4-Mef2.R} P{Mhc-tauGFP} P{UAS-GMA} P{UAS-if.c/g} P{UAS-mCD8::GFP.L} P{UAS-mys.din} P{UAS-slow-HA} P{UAS-sr.isoA} P{UAS-Tsp.AD} P{UAS-TspAD-GFP} P{UAS-TspNTD-GFP} P{XP} PBac{WH}
Genes (13)
	Avic\GFP if Mmus\Cd8a Moe mys rhea Scer\GAL4 shot slow sr T:Avic\GFP T:Ivir\HA1 Tsp
Insertions (6)
	P{en2.4-GAL4}e22c P{GAL4}repo P{GawB}sr^md710 P{GawB}tey^5053A P{XP}d01019 PBac{WH}Syx17^f03584
Natural transposons (1)
	P-element