Reference Report

Reference
Citation	Hamanaka, Y., Park, D., Yin, P., Annangudi, S.P., Edwards, T.N., Sweedler, J., Meinertzhagen, I.A., Taghert, P.H. (2010). Transcriptional Orchestration of the Regulated Secretory Pathway in Neurons by the bHLH protein DIMM. Curr. Biol. 20(1): 9--18. (Export to RIS)
FlyBase ID	FBrf0209946
Publication Type	Research paper
PubMed ID	20045330
PubMed Abstract	The Drosophila basic helix-loop-helix (bHLH) gene dimmed (dimm) promotes a neurosecretory/neuroendocrine phenotype in cells but is not associated with specific neuropeptides or neurohormones. Rather, it is expressed by those peptidergic neurons that project long axons and appear to produce large amounts of secretory peptides. Here, we genetically transform nonpeptidergic neurons in Drosophila to study DIMM's action mechanisms.Nonpeptidergic neurons normally fail to accumulate ectopic neuropeptides. We now show that they will do so when they are also forced to express ectopic DIMM. Furthermore, mass spectrometry shows that photoreceptors, which are normally nonpeptidergic, fail to process an ectopic neuropeptide precursor to make bioactive peptides but will do so efficiently when DIMM is co-misexpressed. Likewise, photoreceptors, which normally package the fast neurotransmitter histamine within small clear synaptic vesicles, produce numerous large dense-core vesicles (LDCVs) when they misexpress DIMM. These novel LDCVs accumulate ectopic neuropeptide when photoreceptors co-misexpress a neuropeptide transgene. DIMM-expressing photoreceptors no longer accumulate histamine and lose synaptic organelles critical to their normal physiology.These findings indicate that DIMM suppresses conventional fast neurotransmission and promotes peptidergic neurosecretory properties. We conclude that DIMM normally provides a comprehensive transcriptional control to direct the differentiation of dedicated neuroendocrine neurons.
DOI	10.1016/j.cub.2009.11.065
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Alleles (7)
	dimm^{Scer\UAS.T:Hsap\MYC} Fmrf^Scer\UAS.cHa Pdf^{pp.Scer\UAS.T:Zzzz\MII} Pdf^Scer\UAS.cRa Scer\GAL4^ap-md544 Scer\GAL4^GMR.long Scer\GAL4^GMR.PU
Constructs (6)
	P{GMR-GAL4.U} P{longGMR-GAL4} P{UAS-dimm.MYC} P{UAS-Fmrf.H} P{UAS-Pdf.R} P{UAS-ppMII}
Genes (8)
	chp Csp dimm Fmrf FR Pdf Scer\GAL4 T:Zzzz\MII
Insertions (1)
	P{GawB}ap^md544
Natural transposons (1)
	P-element