Reference Report

Reference
Citation	Kim, Y.I., Ryu, T., Lee, J., Heo, Y.S., Ahnn, J., Lee, S.J., Yoo, O. (2010). A genetic screen for modifiers of Drosophila caspase Dcp-1 reveals caspase involvement in autophagy and novel caspase-related genes. BMC Cell Biol. 11(): 9. (Export to RIS)
FlyBase ID	FBrf0209948
Publication Type	Research paper
PubMed ID	20100334
PubMed Abstract	Caspases are cysteine proteases with essential functions in the apoptotic pathway; their proteolytic activity toward various substrates is associated with the morphological changes of cells. Recent reports have described non-apoptotic functions of caspases, including autophagy. In this report, we searched for novel modifiers of the phenotype of Dcp-1 gain-of-function (GF) animals by screening promoter element- inserted Drosophila melanogaster lines (EP lines).We screened approximately 15,000 EP lines and identified 72 Dcp-1-interacting genes that were classified into 10 groups based on their functions and pathways: 4 apoptosis signaling genes, 10 autophagy genes, 5 insulin/IGF and TOR signaling pathway genes, 6 MAP kinase and JNK signaling pathway genes, 4 ecdysone signaling genes, 6 ubiquitination genes, 11 various developmental signaling genes, 12 transcription factors, 3 translation factors, and 11 other unclassified genes including 5 functionally undefined genes. Among them, insulin/IGF and TOR signaling pathway, MAP kinase and JNK signaling pathway, and ecdysone signaling are known to be involved in autophagy. Together with the identification of autophagy genes, the results of our screen suggest that autophagy counteracts Dcp-1-induced apoptosis. Consistent with this idea, we show that expression of eGFP-Atg5 rescued the eye phenotype caused by Dcp-1 GF. Paradoxically, we found that over-expression of full-length Dcp-1 induced autophagy, as Atg8b-GFP, an indicator of autophagy, was increased in the eye imaginal discs and in the S2 cell line. Taken together, these data suggest that autophagy suppresses Dcp-1-mediated apoptotic cell death, whereas Dcp-1 positively regulates autophagy, possibly through feedback regulation.We identified a number of Dcp-1 modifiers that genetically interact with Dcp-1-induced cell death. Our results showing that Dcp-1 and autophagy-related genes influence each other will aid future investigations of the complicated relationships between apoptosis and autophagy.
DOI	10.1186/1471-2121-11-9
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	BMC Cell Biol.
Title	BMC Cell Biology
Publication Year	2000-
ISBN/ISSN	1471-2121
Data from Reference
Alleles (55)
	Akt1^{Scer\UAS.T:Ivir\HA1} aop^EP598 Ark^Scer\UAS.cKa Atg1^G13748 Atg2^G6691 Atg4^G18114 Atg5^{Scer\UAS.T:Avic\GFP-EGFP} Atg6^G3772 Atg6^G6654 Atg6^G6854 Atg7^G8010 Atg8a^G9749 Atg8b^{hs.T:Avic\GFP} Atg18^G3744 Aut1^G3894 BacA\p35^Scer\UAS.cHa bchs^G2362 bchs^G12113 bchs^G13044 br^G1972 br^G10174 bsk^DN.Scer\UAS Dcp-1^Scer\UAS.cKa Dl^D.Scer\UAS Dredd^Scer\UAS.cKa eff^G15069 Egfr^Scer\UAS.cBa Eip55E^G2166 Eip55E^G13564 Eip74EF^G15347 Eip78C^G14526 esg^EP633 esg^EP2009 faf^EP3520 Hsc70-4^{D206S.Scer\UAS} Nc^Scer\UAS.cKa Pi3K92E^{Scer\UAS.T:Hsap\MYC} psq^G7540 Pten^Scer\UAS.cGa Rac1^N17.Scer\UAS S6k⁰⁷⁰⁸⁴ S6k^Scer\UAS.cMa Scer\GAL4^ap-md544 Scer\GAL4^Bx-MS1096 Scer\GAL4^GMR.PF SNF4Aγ^GX6409 th^EP3279 Tor^k17004 Uba1^G3534 Uba2^G4384 Uba2^G6571 Uba2^G8583 UbcD4^G4874 Ubp64E^G5032 Ubp64E^G5401
Constructs (18)
	P{EP} P{GAL4-ninaE.GMR} P{hs-Atg8b.GFP} P{UAS-Akt1.HA} P{UAS-Ark.K} P{UAS-bsk.DN} P{UAS-Dcp-1.K} P{UAS-Dl.DN} P{UAS-Dredd.K} P{UAS-eGFP-drAtg5} P{UAS-Egfr.B} P{UAS-Hsc70-4.D206S} P{UAS-Nc.K} P{UAS-p35.H} P{UAS-Pten.G} P{UAS-Rac1.N17} P{UAS-S6k.M} P{WT-Dp110}
Genes (50)
	Akt1 aop Ark Atg1 Atg2 Atg4 Atg5 Atg6 Atg7 Atg8a Atg8b Atg18 Aut1 BacA\p35 bchs br bsk CG10969 Dcp-1 Dl Dredd eff Egfr Eip55E Eip74EF Eip78C esg faf hep Hsc70-4 InR Mekk1 mkp Mkp Nc Pi3K92E psq Pten Rac1 S6k Scer\GAL4 SNF4Aγ Tak1 Tango5 th Tor Uba1 Uba2 UbcD4 Ubp64E
Insertions (47)
	P{EP}aop^EP598 P{EP}Atg1^G13748 P{EP}Atg2^G6691 P{EP}Atg4^G18114 P{EP}Atg6^G3772 P{EP}Atg6^G6654 P{EP}Atg6^G6854 P{EP}Atg7^G8010 P{EP}Atg8a^G9749 P{EP}Atg18^G3744 P{EP}Aut1^G3894 P{EP}bchs^G2362 P{EP}bchs^G12113 P{EP}bchs^G13044 P{EP}br^G1972 P{EP}br^G10174 P{EP}eff^G15069 P{EP}Eip55E^G2166 P{EP}Eip55E^G13564 P{EP}Eip74EF^G15347 P{EP}Eip78C^G14526 P{EP}esg^EP633 P{EP}esg^EP2009 P{EP}faf^EP3520 P{EP}psq^G7540 P{EP}SNF4Aγ^GX6409 P{EP}th^EP3279 P{EP}Uba1^G3534 P{EP}Uba2^G4384 P{EP}Uba2^G6571 P{EP}Uba2^G8583 P{EP}UbcD4^G4874 P{EP}Ubp64E^G5032 P{EP}Ubp64E^G5401 P{GawB}ap^md544 P{GawB}Bx^MS1096 P{lacW}Tor^k17004 P{PZ}S6k⁰⁷⁰⁸⁴ P{UAS-Dcp-1.K}1-1 P{UAS-Dcp-1.K}1-2 P{UAS-Dcp-1.K}1-4 P{UAS-Dcp-1.K}1-5 P{UAS-Dcp-1.K}2-4 P{UAS-Dcp-1.K}4-1 P{UAS-Dcp-1.K}5-4 P{UAS-Dcp-1.K}7-3 P{UAS-Dcp-1.K}19-2
Natural transposons (1)
	P-element