Reference Report

Reference
Citation	Owald, D., Fouquet, W., Schmidt, M., Wichmann, C., Mertel, S., Depner, H., Christiansen, F., Zube, C., Quentin, C., Körner, J., Urlaub, H., Mechtler, K., Sigrist, S.J. (2010). A Syd-1 homologue regulates pre- and postsynaptic maturation in Drosophila. J. Cell Biol. 188(4): 565--579. (Export to RIS)
FlyBase ID	FBrf0210057
Publication Type	Research paper
PubMed ID	20176924
PubMed Abstract	Active zones (AZs) are presynaptic membrane domains mediating synaptic vesicle fusion opposite postsynaptic densities (PSDs). At the Drosophila neuromuscular junction, the ELKS family member Bruchpilot (BRP) is essential for dense body formation and functional maturation of AZs. Using a proteomics approach, we identified Drosophila Syd-1 (DSyd-1) as a BRP binding partner. In vivo imaging shows that DSyd-1 arrives early at nascent AZs together with DLiprin-alpha, and both proteins localize to the AZ edge as the AZ matures. Mutants in dsyd-1 form smaller terminals with fewer release sites, and release less neurotransmitter. The remaining AZs are often large and misshapen, and ectopic, electron-dense accumulations of BRP form in boutons and axons. Furthermore, glutamate receptor content at PSDs increases because of excessive DGluRIIA accumulation. The AZ protein DSyd-1 is needed to properly localize DLiprin-alpha at AZs, and seems to control effective nucleation of newly forming AZs together with DLiprin-alpha. DSyd-1 also organizes trans-synaptic signaling to control maturation of PSD composition independently of DLiprin-alpha.
DOI	10.1083/jcb.200908055
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (3)
	Df(2R)BSC29 Df(3R)RhoGAP100F^ex1.2 Df(3R)RhoGAP100F^ex3.4
Alleles (18)
	brp⁶⁹ brp^{S.Scer\UAS.T:Disc\RFP-mStraw} gfA^{Scer\UAS.T:Avic\GFP-EGFP} heph^ex3.4 Liprin-α^F3ex15 Liprin-α^R60 Liprin-α^{Scer\UAS.T:Avic\GFP-EGFP} Liprin-α^{Scer\UAS.T:Avic\GFP-YFP} RhoGAP100F^Scer\UAS.cOa RhoGAP100F^{Scer\UAS.T:Avic\GFP-EGFP} RhoGAP100F^{Scer\UAS.T:Disc\RFP-mStraw} Scer\GAL4^D42 Scer\GAL4^elav.PU Scer\GAL4^ey-OK107 Scer\GAL4^G14 Scer\GAL4^Rapgap1-OK6 T:Avic\GFP^EGFP T:Disc\RFP^mStraw
Constructs (7)
	P{elav-GAL4.U} P{UAS-brp.S-mStraw} P{UAS-Liprin-α.EGFP} P{UAS-Liprin-α.YFP} P{UAS-RhoGAP100F.EGFP} P{UAS-RhoGAP100F.mStraw} P{UAS-RhoGAP100F.O}
Genes (11)
	brp ferrochelatase gfA GluRIIA GluRIIB GluRIID heph Liprin-α RhoGAP100F Scer\GAL4 VGlut
Insertions (8)
	P{GAL4}G14 P{GawB}D42 P{GawB}ey^OK107 P{GawB}OK6 P{XP}d10862 P{XP}heph^d09905 PBac{RB}e04118 PBac{WH}f02651
Natural transposons (1)
	P-element