Reference Report

Reference
Citation	Gao, G., Walser, J.C., Beaucher, M.L., Morciano, P., Wesolowska, N., Chen, J., Rong, Y.S. (2010). HipHop interacts with HOAP and HP1 to protect Drosophila telomeres in a sequence-independent manner. EMBO J. 29(4): 819--829. (Export to RIS)
FlyBase ID	FBrf0210059
Publication Type	Research paper
PubMed ID	20057353
PubMed Abstract	Telomeres prevent chromosome ends from being repaired as double-strand breaks (DSBs). Telomere identity in Drosophila is determined epigenetically with no sequence either necessary or sufficient. To better understand this sequence-independent capping mechanism, we isolated proteins that interact with the HP1/ORC-associated protein (HOAP) capping protein, and identified HipHop as a subunit of the complex. Loss of one protein destabilizes the other and renders telomeres susceptible to fusion. Both HipHop and HOAP are enriched at telomeres, where they also interact with the conserved HP1 protein. We developed a model telomere lacking repetitive sequences to study the distribution of HipHop, HOAP and HP1 using chromatin immunoprecipitation (ChIP). We discovered that they occupy a broad region >10 kb from the chromosome end and their binding is independent of the underlying DNA sequence. HipHop and HOAP are both rapidly evolving proteins yet their telomeric deposition is under the control of the conserved ATM and Mre11-Rad50-Nbs (MRN) proteins that modulate DNA structures at telomeres and at DSBs. Our characterization of HipHop and HOAP reveals functional analogies between the Drosophila proteins and subunits of the yeast and mammalian capping complexes, implicating conservation in epigenetic capping mechanisms.
DOI	10.1038/emboj.2009.394
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Aberrations (1)
	Df(2R)D4A^TD
Alleles (8)
	cav¹ cav^T:Hsap\MYC mre11^Δ35K1 nbs^Δ180 Scer\SCEI^hs.PR tefu^stg w^+mW.I-SceI w^D4A-TD
Constructs (3)
	P{70I-SceI} P{w^+mW.I-SceI} P{w^+mW.-I-SceI}
Genes (13)
	Avic\GFP Cat cav CG32795 HipHop His3 mre11 nbs Scer\SCEI Su(var)205 T:Hsap\MYC tefu w
Insertions (2)
	P{w^+mW.I-SceI}D4A P{w^+mW.-I-SceI}D4A-TD
Natural transposons (2)
	HeT-A P-element