Reference Report

Reference
Citation	Fichelson, P., Moch, C., Ivanovitch, K., Martin, C., Sidor, C.M., Lepesant, J.A., Bellaiche, Y., Huynh, J.R. (2009). Live-imaging of single stem cells within their niche reveals that a U3snoRNP component segregates asymmetrically and is required for self-renewal in Drosophila. Nat. Cell Biol. 11(6): 685--693. (Export to RIS)
FlyBase ID	FBrf0210070
Publication Type	Research paper
PubMed ID	19430468
PubMed Abstract	Stem cells generate self-renewing and differentiating progeny over many rounds of asymmetric divisions. How stem cell growth rate and size are maintained over time remains unknown. We isolated mutations in a Drosophila melanogaster gene, wicked (wcd), which induce premature differentiation of germline stem cells (GSCs). Wcd is a member of the U3 snoRNP complex required for pre-ribosomal RNA maturation. This general function of Wcd contrasts with its specific requirement for GSC self-renewal. However, live imaging of GSCs within their niche revealed a pool of Wcd-forming particles that segregate asymmetrically into the GSCs on mitosis, independently of the Dpp signal sent by the niche. A fraction of Wcd also segregated asymmetrically in dividing larval neural stem cells (NSCs). In the absence of Wcd, NSCs became smaller and produced fewer neurons. Our results show that regulation of ribosome synthesis is a crucial parameter for stem cell maintenance and function.
DOI
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Supplementary material	Online methods. [FBrf0209977]
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Cell Biol.
Title	Nature Cell Biology
Publication Year	1999-
ISBN/ISSN	1465-7392 1476-4679
Data from Reference
Alleles (9)
	BacA\p35^Scer\UAS.cHa Hsap\HIST2H2BE^{Scer\UAS.T:Disc\RFP-mRFP} Scer\GAL4^{nos.UTR.T:Hsim\VP16} tkv^{Q199D.Scer\UAS.P\T} wcd¹ wcd³ wcd^k07824 wcd^{Scer\UAS.P\T.T:Avic\GFP-EGFP} wcd^{Scer\UAS.P\T.T:Disc\RFP-mRFP}
Constructs (6)
	P{GAL4::VP16-nos.UTR} P{UAS-Hsap\H2BFQ.mRFP} P{UAS-p35.H} P{UASp-tkv.Q199D} P{UAS-RFP-wcd} P{UAS-wcd-GFP}
Genes (17)
	18SrRNA 28SrRNA BacA\p35 bb Fib His3 Hsap\HIST2H2BE Lsm11 mira orb Scer\GAL4 snoRNA:U3:9B snoRNA:U3:54Aa snoRNA:U3:54Ab tkv wcd α-Spec
Insertions (1)
	P{lacW}wcd^k07824
Natural transposons (1)
	P-element